收稿日期: 2020-04-21
网络出版日期: 2020-09-09
基金资助
广西科技重大专项(桂科 No.AA17204057)
Investigation of Fasciola spp. infection in Galba pervia and buffaloes in Guangxi
Received date: 2020-04-21
Online published: 2020-09-09
Supported by
Science and Technology Major Project of Guangxi(桂科 No.AA17204057)
目的 了解广西片形吸虫中间宿主小土蜗和终宿主水牛的感染情况。方法 2018年4-10月,于广西横县、宾阳、隆安、都安、大化和来宾等6个县（市）收集小土蜗和水牛粪便,小土蜗通过压片镜检查找幼虫,水牛粪样采用水洗沉淀法查找虫卵,对发现的片形吸虫幼虫和虫卵提取DNA,进行内转录间隔区2（ITS2）序列PCR扩增和鉴定。结果 共采集1 523只小土蜗,片形吸虫平均感染率为0.3%（5/1 523）,除大化县和都安县采集的小土蜗未发现片形吸虫感染外,横县、宾阳、隆安、来宾等4个县（市）采集的小土蜗均存在片形吸虫感染。共采集128份水牛粪样,平均感染率为79.7%（102/128）。6个县（市）水牛均存在片形吸虫感染,其中来宾市水牛感染率最高,为88.2%（15/17）;都安县水牛感染率最低,为64.0%（16/25）。片形吸虫ITS2序列PCR扩增长度约为500 bp。小土蜗中片形吸虫幼虫ITS2序列与巨片形吸虫的ITS2序列（GenBank登录号为KF667375.1）的相似性为98.8%~99.0%,水牛粪样中片形吸虫虫卵ITS2序列与巨片形吸虫的ITS2序列（GenBank登录号为KF667375.1）的相似性为98.4%~99.4%。结论 广西相关调查点的小土蜗和水牛均存在巨片形吸虫感染,人群存在感染风险,应加强防控。
梁安莉 , 农珍妮 , 卢秀红 , 石云良 , 张为宇 . 广西小土蜗和水牛片形吸虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2020 , 38(4) : 449 -453 . DOI: 10.12140/j.issn.1000-7423.2020.04.009
Objective To investigate the status of Fasciola spp. infection in the intermediate host Galba pervia and the definitive host buffaloes.Methods From April to October 2018, Galba pervia snails and buffalo feces were collected in six counties(cities) of Guangxi Province, including Hengxian County, Binyang County, Long’an County, Du’an County, Dahua County and Laibin County. The snails underwent compressed smear microscopy to observe worm larvae, and the fecal samples were examined using washing-sedimentation method for eggs. The DNA extracted from the larvae and eggs of Fasciola were processed for ITS2(internal transcribed spacer 2, ITS2) amplification and identification.Results A total of 1 523 snails were collected, showing an average Fasciola infection rate of 0.3% (5/1 523). Fasciola infection was found in the snail samples from four counties of Hengxian, Binyang, Long’an, and Laibin, but not from the Du’an and Dahua counties. A total of 128 buffalo fecal samples were collected, displaying an average Fasciola infection rate of 79.7% (102/128). The buffaloes from all six counties were found Fasciola egg positive in feces, with the Laibin County having the highest infection rate(88.2%, 15/17), and Du’an County the lowest rate (64.0%, 16/25). The sequence length of ITS2 amplification products was about 500 bp. The ITS2 amplification product from larvae in the snails had sequence homology of 98.8%-99.0% to that of F. gigantica (GenBank accession no. KF667375.1); the amplified sequence from fecal eggs of water buffaloes showed 98.4%-99.4% similarity to that of F. gigantica (GenBank accession no. KF667375.1).Conclusion The survey indicates that F. gigantica infections were found in snail Galba pervia and buffaloes in Guangxi, suggesting potential risk of human infection, thus, it is imperative to strengthen prevention and control measures.
Key words： Fasciola spp.; Galba pervia; Buffalo; Guangxi
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