收稿日期: 2019-10-14
网络出版日期: 2020-07-07
基金资助
科技部重点研发计划项目(2017YFD0500400);国家自然科学基金(31672548)
Research progress on the in vitro culture model of Cryptosporidium species
Received date: 2019-10-14
Online published: 2020-07-07
Supported by
National Key Research and Development Program of China(2017YFD0500400);National Natural Science Foundation of China(31672548)
牛子文 , 常艳凯 , 王克 , 张龙现 . 隐孢子虫体外培养模型研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2020 , 38(3) : 394 -399 . DOI: 10.12140/j.issn.1000-7423.2020.03.022
Cryptosporidium is an important zoonotic protozoan parasite, which mainly parasitizes in intestinal epithelial cells, causing diarrhea in humans and animals. To date, there are no effective drugs or vaccines for control of cryptosporidiosis. Establishing an efficient culture model may provide experimental basis for studying the mechanisms of host cell invasion by Cryptosporidium and the development of vaccines and drug against Cryptosporidium. Comparing to common animal infection models, in vitro culture system has advantages of low cost and being able to simulate physiological status of hosts, compared to common animal models of infection, thus facilitating anti-Cryptosporidium drug screen. This article reviews the research progress on chicken embryo and chicken embryo organ culture models, the cell culture models, the cell-free culture model, the three-dimensional culture model and intestinal organoids culture model for Cryptosporidium, to provide reference for further understanding of pathogenic mechanisms of Cryptosporidium and the development of anti-Cryptosporidium drugs.
Key words： Cryptosporidium; In vitro culture model; Research progress
| [1] | Zheng SJ, Li DF, Zhou CX, et al. Molecular identification and epidemiological comparison of Cryptosporidium spp. among different pig breeds in Tibet and Henan, China[J]. BMC Vet Res, 2019,15(1):101. |
| [2] | Checkley W, White AC Jr, Jaganath D, et al. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for Cryptosporidium[J]. Lancet Infect Dis, 2015,15(1):85-94. |
| [3] | Feng YY, Ryan UM, Xiao LH. Genetic diversity and population structure of Cryptosporidium[J]. Trends Parasitol, 2018,34(11):997-1011. |
| [4] | Jia D, Dan JM, Sun Y, et al. Prevalence and molecular characteristics of Cryptosporidium infection in pre-weaned dairy calves in Sichuan Province[J]. Chin J Parasitol Parasit Dis, 2019,37(4):422-427. (in Chinese) |
| [4] | ( 贾丁, 但佳明, 孙燕, 等. 四川省奶牛犊牛隐孢子虫流行情况及其分子特性分析[J]. 中国寄生虫学与寄生虫病杂志, 2019,37(4):422-427.) |
| [5] | Li WC, Wang K, Tang L, et al. Molecular characterization of Cryptosporidium species in sheep and goats in Anhui Province and neighboring Provinces[J]. Chin J Schisto Control, 2019,31(5):474-478, 485. (in Chinese) |
| [5] | ( 李文超, 汪凯, 唐莉, 等. 安徽及周边省份绵羊和山羊隐孢子虫分子特性分析[J]. 中国血吸虫病防治杂志, 2019,31(5):474-478, 485.) |
| [6] | Sheoran AS, Pina-Mimbela R, Keleher A, et al. Infection with anthroponotic Cryptosporidium parvum does not fully protect the host against a subsequent challenge with C. hominis[J]. Microbes Infect, 2018,20(4):267-270. |
| [7] | Villanueva MT. Infectious diseases: decrypting Cryptosporidium[J]. Nat Rev Drug Discov, 2017,16(8):527. |
| [8] | Huang DN, Zhang RL, Tang YJ, et al. Establishment and application of PCR method for detection of Cryptosporidium[J]. China Trop Med, 2016,16(8):770-773. (in Chinese) |
| [8] | ( 黄达娜, 张仁利, 唐屹君, 等. 隐孢子虫PCR检测体系的建立及其应用[J]. 中国热带医学, 2016,16(8):770-773.) |
| [9] | Sateriale A, ?lapeta J, Baptista R, et al. A genetically tractable, natural mouse model of cryptosporidiosis offers insights into host protective immunity[J]. Cell Host Microbe, 2019,26(1):135-146. |
| [10] | Ch Stratakos A, Sima F, Ward P, et al. The in vitro and ex vivo effect of Auranta 3001 in preventing Cryptosporidium hominis and Cryptosporidium parvum infection[J]. Gut Pathog, 2017,9:49. |
| [11] | Liu L, Johnson HL, Cousens S, et al. Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000[J]. Lancet, 2012,379(9832):2151-2161. |
| [12] | Gharpure R, Perez A, Miller AD, et al. Cryptosporidiosis outbreaks - United States, 2009-2017[J]. MMWR Morb Mortal Wkly Rep, 2019,68(25):568-572. |
| [13] | Ming Z, Zhou R, Chen XM. Regulation of host epithelial responses to Cryptosporidium infection by microRNAs[J]. Parasite Immunol, 2017,39(2). |
| [14] | King P, Tyler KM, Hunter PR. Anthroponotic transmission of Cryptosporidium parvum predominates in countries with poorer sanitation: a systematic review and meta-analysis[J]. Parasit Vectors, 2019,12(1):16. |
| [15] | Li SR, Niu ZW, Zhang SM, et al. Advances in the study of and uses of animal models of Cryptosporidium parvum infection[J]. J Pathog Biol, 2018,13(7):785-788. (in Chinese) |
| [15] | ( 李松瑞, 牛子文, 张素梅, 等. 微小隐孢子虫感染动物模型及其应用研究进展[J]. 中国病原生物学杂志, 2018,13(7):785-788.) |
| [16] | Karanis P. The truth about in vitro culture of Cryptosporidium species[J]. Parasitology, 2018,145(7):855-864. |
| [17] | Paziewska-Harris A, Schoone G, Schallig HDFH. Long-term storage of Cryptosporidium parvum for in vitro culture[J]. J Parasitol, 2018,104(1):96-100. |
| [18] | Current WL, Long PL. Development of human and calf Cryptosporidium in chicken embryos[J]. J Infect Dis, 1983,148(6):1108-1113. |
| [19] | Lindsay DS, Sundermann CA, Blagburn BL. Cultivation of Cryptosporidium baileyi: studies with cell cultures, avian embryos, and pathogenicity of chicken embryo-passaged oocysts[J]. J Parasitol, 1988,74(2):288-293. |
| [20] | Zhang XC, Hu LS, Li DC. Cryptosporidium of rabbits were successfully cultured on chicken embryos[J]. Veterinary Coll PLA, 1990,10(2):117. (in Chinese) |
| [20] | ( 张西臣, 胡力生, 李德昌. 兔的隐孢子虫在鸡胚上培养成功[J]. 兽医大学学报, 1990,10(2):117.) |
| [21] | Zhao YR, Song SG, Zhang JY, et al. The life-cycle of Cryptosporidium baileyi in the cam of chicken embryo[J]. Sci Agricutura Sin, 1995, 28(6): 83-89, 95-96. (in Chinese) |
| [21] | ( 赵亚荣, 宋生贵, 张晋英, 等. 贝氏隐孢子虫在鸡胚绒毛尿囊膜上发育过程的研究[J]. 中国农业科学, 1995, 28(6): 83-89, 95-96.) |
| [22] | Zhang SM, Zhao JF, Huang L, et al. Evaluation of curative anticryptosporidial activity of four kinds of potential drugs in chick embryo and chicken[J]. Chin J Vet Sci, 2011,31(1):59-64. (in Chinese) |
| [22] | ( 张素梅, 赵金凤, 黄磊, 等. 贝氏隐孢子虫鸡胚和雏鸡增殖模型筛选抗隐孢子虫药物试验[J]. 中国兽医学报, 2011,31(1):59-64.) |
| [23] | Huang L. Developmental history of Cryptosporidium baileyi in chicken embryos and screening of anti-Cryptosporidium drugs[D]. Zhengzhou: Henan Agricultural University, 2010. (in Chinese) |
| [23] | ( 黄磊. 贝氏隐孢子虫在鸡胚中发育史及抗隐孢子虫药物筛选研究[D]. 郑州: 河南农业大学, 2010.) |
| [24] | Huang L, Zhang Y, Zhang SM, et al. Cryptosporidium baileyi oocysts cultured in chicken embryo[J]. Chin J Zoonoses, 2012,28(3):205-208. (in Chinese) |
| [24] | ( 黄磊, 张云, 张素梅, 等. 贝氏隐孢子虫鸡胚培养方法研究[J]. 中国人兽共患病学报, 2012,28(3):205-208.) |
| [25] | Huang L, Zhu HL, Zhang SM, et al. An in vitro model of infection of chicken embryos by Cryptosporidium baileyi[J]. Exp Parasitol, 2014,147:41-47. |
| [26] | Ren GJ. Study on expression profiles of lncRNA and circRNA in tracheas of chickens infected with Cryptosporidium baileyi[D]. Yangling, China: Northwest A & F University, 2018. (in Chinese) |
| [26] | ( 任冠静. 贝氏隐孢子虫感染雏鸡气管的lncRNA与circRNA的表达谱研究[D]. 杨凌: 西北农林科技大学, 2018.) |
| [27] | Zhang SM, Jian FC, Zhao GH, et al. Chick embryo tracheal organ: a new and effective in vitro culture model for Cryptosporidium baileyi[J]. Vet Parasitol, 2012,188(3/4):376-381. |
| [28] | Woodmansee DB, Pohlenz JFL. Development of Cryptosporidium sp. in a human rectal tumor cell line [C]. Saskatoon: Proceedings of the Fourth International Symposium on Neonatal Diarrhea, 1983: 306-319. |
| [29] | Current WL, Haynes TB. Complete development of Cryptosporidium in cell culture[J]. Science, 1984,224(4649):603-605. |
| [30] | Woodmansee DB. An in vitro study of sporulation in Cryptosporidium species[J]. J Parasitol, 1986,72(2):348-349. |
| [31] | Flanigan TP, Aji T, Marshall R, et al. Asexual development of Cryptosporidium parvum within a differentiated human enterocyte cell line[J]. Infect Immun, 1991,59(1):234-239. |
| [32] | Rosales MJ, Cifuentes J, Mascaró C. Cryptosporidium parvum: culture in MDCK cells[J]. Exp Parasitol, 1993,76(2):209-212. |
| [33] | Yu JR, Choi SD, Kim YW. In vitro infection of Cryptosporidium parvum to four different cell lines[J]. Korean J Parasitol, 2000,38(2):59-64. |
| [34] | Chen F, Huang KH. In vitro cultivation model of Cryptosporidium parvum in MDCK cells and its development[J]. Chin J Parasitol Parasit Dis, 2009,27(3):219-222. (in Chinese) |
| [34] | ( 陈甫, 黄克和. 微小隐孢子虫感染犬肾细胞模型的建立及生长发育过程的研究[J]. 中国寄生虫学与寄生虫病杂志, 2009,27(3):219-222.) |
| [35] | Upton SJ, Tilley M, Brillhart DB. Comparative development of Cryptosporidium parvum (Apicomplexa) in 11 continuous host cell lines[J]. FEMS Microbiol Lett, 1994,118(3):233-236. |
| [36] | Hijjawi NS, Meloni BP, Morgan UM, et al. Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture[J]. Int J Parasitol, 2001,31(10):1048-1055. |
| [37] | Wu L, Chen SX, Jiang XG, et al. Effect of select medium supplements on in vitro development of Cryptosporidium andersoni in HCT-8 cells[J]. Parasitol Res, 2009,105(5):1419-1424. |
| [38] | Miller CN, Jossé L, Brown I, et al. A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology[J]. Int J Parasitol, 2018,48(3/4):197-201. |
| [39] | Castellanos-Gonzalez A, Cabada MM, Nichols J, et al. Human primary intestinal epithelial cells as an improved in vitro model for Cryptosporidium parvum infection[J]. Infect Immun, 2013,81(6):1996-2001. |
| [40] | Varughese EA, Bennett-Stamper CL, Wymer LJ, et al. A new in vitro model using small intestinal epithelial cells to enhance infection of Cryptosporidium parvum[J]. J Microbiol Methods, 2014,106:47-54. |
| [41] | Hijjawi NS, Meloni BP, Ng'anzo M, et al. Complete development of Cryptosporidium parvum in host cell-free culture[J]. Int J Parasitol, 2004,34(7):769-777. |
| [42] | Boxell A, Hijjawi N, Monis P, et al. Comparison of various staining methods for the detection of Cryptosporidium in cell-free culture[J]. Exp Parasitol, 2008,120(1):67-72. |
| [43] | Hijjawi N, Estcourt A, Yang RC, et al. Complete development and multiplication of Cryptosporidium hominis in cell-free culture[J]. Vet Parasitol, 2010,169(1/2):29-36. |
| [44] | Yang R, Elankumaran Y, Hijjawi N, et al. Validation of cell-free culture using scanning electron microscopy (SEM) and gene expression studies[J]. Exp Parasitol, 2015,153:55-62. |
| [45] | Aldeyarbi HM, Karanis P. Electron microscopic observation of the early stages of Cryptosporidium parvum asexual multiplication and development in in vitro axenic culture[J]. Eur J Protistol, 2016,52:36-44. |
| [46] | Aldeyarbi HM, Karanis P. The fine structure of sexual stage development and sporogony of Cryptosporidium parvum in cell-free culture[J]. Parasitology, 2016,143(6):749-761. |
| [47] | Aldeyarbi HM, Karanis P. The ultra-structural similarities between Cryptosporidium parvum and the gregarines[J]. J Eukaryot Microbiol, 2016,63(1):79-85. |
| [48] | Alcantara Warren C, Destura RV, Sevilleja JE, et al. Detection of epithelial-cell injury, and quantification of infection, in the HCT-8 organoid model of cryptosporidiosis[J]. J Infect Dis, 2008,198(1):143-149. |
| [49] | Morada M, Lee S, Gunther-Cummins L, et al. Continuous culture of Cryptosporidium parvum using hollow fiber technology[J]. Int J Parasitol, 2016,46(1):21-29. |
| [50] | DeCicco RePass MA, Chen Y, Lin YN, et al. Novel bioengineered three-dimensional human intestinal model for long-term infection of Cryptosporidium parvum[J]. Infect Immun, 2017,85(3):e00731-e00716. |
| [51] | Wilke G, Funkhouser-Jones LJ, Wang Y, et al. A stem-cell-derived platform enables complete Cryptosporidium development in vitro and genetic tractability[J]. Cell Host Microbe, 2019,26(1):123-134. |
| [52] | Kunz KT, Przysiecki N, Effenberger M, et al. Crohn's disease (CD) derived intestinal organoids as a model to study inflammation emanating from the intestinal epithelium[J]. Z Gastroenterol, 2019,57(05):e130-e131. |
| [53] | Nakamura T. Recent progress in organoid culture to model intestinal epithelial barrier functions[J]. Int Immunol, 2019,31(1):13-21. |
| [54] | Drost J, Clevers H. Organoids in cancer research[J]. Nat Rev Cancer, 2018,18(7):407-418. |
| [55] | Flatres C, Loffet é, Neunlist M, et al. From human pluripotent stem cells to custom-made intestinal organoids[J]. Med Sci (Paris), 2019,35(6/7):549-555. |
| [56] | Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche[J]. Nature, 2009,459(7244):262-265. |
| [57] | Sato T, Stange DE, Ferrante M, et al. Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium[J]. Gastroenterology, 2011,141(5):1762-1772. |
| [58] | Augustyniak J, Bertero A, Coccini T, et al. Organoids are promising tools for species-specific in vitro toxicological studies[J]. J Appl Toxicol, 2019,39(12):1610-1622. |
| [59] | Klotz C, Aebischer T, Seeber F. Stem cell-derived cell cultures and organoids for protozoan parasite propagation and studying host-parasite interaction[J]. Int J Med Microbiol, 2012,302(4/5):203-209. |
| [60] | Baydoun M, Vanneste SB, Creusy C, et al. Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: proof of concept[J]. Sci Rep, 2017,7(1):17288. |
| [61] | Heo I, Dutta D, Schaefer DA, et al. Modelling Cryptosporidium infection in human small intestinal and lung organoids[J]. Nat Microbiol, 2018,3(7):814-823. |
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