应用斑点免疫金渗滤法检测旋毛虫病患者血清IgG

中国寄生虫学与寄生虫病杂志 ›› 2003, Vol. 21 ›› Issue (6): 7-344.

应用斑点免疫金渗滤法检测旋毛虫病患者血清IgG

刘英杰,刘云,曲耀华,郑勇,刘艳红,张永利,陈娟

河南大学医学院细胞与分子免疫学实验室,开封 475001 (刘英杰;刘云;曲耀华),河南大学第一附属医院,河南开封 475001 (郑勇),开封市防疫站,开封 475000 (刘艳红),河南大学附属淮河医院,开封 475000 (张永利)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-12-30 发布日期:2003-12-30

Application of Dot-immunogold Filtration Assay in Detection of IgG in Sera of Trichinellosis Patients

LIU Ying-jie~1,LIU Yun~1,QU Yao-hua~1,ZHENG Yong~2,LIU Yan-hong~3,ZHANG Yong-li~4,CHEN Juan~3

1 Cellular and Molecular Immunology Laboratory of Henan University,Kaifeng 475001 2 The First Affiliated Hospital of Henan University,Kaifeng 475001 3 Kaifeng Epidemic Prevention Station,Kaifeng 475000 4 Huaihe Affiliated Hospital of Henan University,Kaifeng 475000

Received:1900-01-01 Revised:1900-01-01 Online:2003-12-30 Published:2003-12-30

摘要/Abstract

摘要： 　　目的　研究应用斑点免疫金渗滤法(DIGFA)快速检测旋毛虫病患者血清抗旋毛虫IgG抗体。　方法　采用旋毛虫肌肉期幼虫膜抗原,以胶体金颗粒结合的羊抗人IgG为标记抗体,以颜色深浅为判断阳性标准。　结果　纯化的旋毛虫肌肉期幼虫膜抗原与患者血清中特异性抗旋毛虫IgG抗体通过渗滤在硝酸纤维素膜上反应,10min内即可直接观察结果。在76份患者血清的检测中,阳性率为94.74%,与ELISA法的检出阳性率86.84%相比差异无显著性(χ2=2.83,P>0.05)。交叉试验与重复试验结果显示DIGFA具有较好的特异性及稳定性。　结论　DIGFA法可快速检测旋毛虫病患者血清特异性旋毛虫IgG抗体

关键词: 旋毛虫病, 斑点免疫金渗滤试验, 酶联免疫吸附试验, 膜抗原, 免疫诊断

Abstract: 　Objective To study a new quick method for detecting serum IgG against Trichinella spiralis (T.s). Methods Membrane antigen of muscle larva of T.s was isolated and combined with the nitrocellulose membrane. Goat anti-human IgG was conjugated with the golden pellets of colloidal state which was used as marked antibody for the experiment of dot-immunogold filtration assay to examine specific IgG. Results T.s IgG and its corresponding antigen reacted on the membrane by filtration, and the result could be observed with naked eyes within 10 min. In the examination of 76 clinical serum samples, the positive rate was (94.7%). It was not significantly higher than that of ELISA(χ~2=2.83,P>0.05). Conclusion The dot-immunogold filtration assay is rapid and simple for performing and reading in the detection of IgG against T.s with high sensitivity and specificity.

Key words: trichinellosis, dot-immunogold filtration, ELISA, membrane antigen, immunodiagnosis

刘英杰;刘云;曲耀华;郑勇;刘艳红;张永利;陈娟. 应用斑点免疫金渗滤法检测旋毛虫病患者血清IgG[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(6): 7-344.

LIUYing-jie~;LIUYun~;QUYao-hua~;ZHENGYong~;LIUYan-hong~;ZHANGYong-li~;CHENJuan~. Application of Dot-immunogold Filtration Assay in Detection of IgG in Sera of Trichinellosis Patients[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(6): 7-344.

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