摘要： Specimens were collected from forehead,nosetip,nasal grooves and chin areas of suspectedindividuals by pressing with both thumbs.Specimens exceeding 20 active Demodex folliculorumwere taken into account(Df),and the number of D.brevis(Db)was counted simultaneouly.10％,5％,1％and 0.5％ amitraz in peanut oil were paepared and used for in vitro test.This test was