中国寄生虫学与寄生虫病杂志 ›› 1993, Vol. 11 ›› Issue (3): 168-171.

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印度雕蚀菌感染三带喙库蚊幼虫的组织化学研究

孙建华,汪政仪,连惟能,刘素兰   

  1. 上海医科大学寄生虫学教研室
  • 出版日期:1993-08-31 发布日期:2017-01-06
  • 基金资助:
    国家自然科学基金(项目编号:3870673)

STUDIES ON THE HISTOCHEMISTRY OF CULEX TRITAENIORHYNCHVS LARVAE INFECTED WITH COELOMOMYCES INDICA

  • Online:1993-08-31 Published:2017-01-06

摘要: 本文采用组织化学技术,对印度雕蚀菌感染的三带喙库蚊幼虫的糖原、蛋白质、核酸(DNA、RNA)进行显微摄影及组成定量的图像分析。结果表明感染的幼蚊组织中糖原、蛋白质、核酸的含量均明显地较正常的幼蚊为少,灰度定量测定也表明两组幼蚊之间的差别非常显著。提示该菌感染对幼蚊上述生化成分有明显的影响,可能是其对幼蚊致病的重要机制。

关键词: 印度雕蚀菌, 三带喙库蚊, 组织化学

Abstract: A sectional survey with histochemical technique was carried out on Culex tritae-niorhynchus larvae infected with Coelomomyces indica in comparison to the noninfected larvae. Studies were pursued by using micrograph and imaging analysis. The results showed that the glycogen, protein and nucleic acid (RNA and DNA)reaction in the infected group were less than those of the control group. The gray level assessment in tissue imaging showed marked difference between the two groups. It is suggested that C. indica has significant effect on the above biochemical elements of the mosquito larvae, which might be considered an important mechanism in the pathogenicity of the fungus.