红带锥蝽实验条件下发育特征研究

doi:10.12140/j.issn.1000-7423.2020.02.014

中国寄生虫学与寄生虫病杂志 ›› 2020, Vol. 38 ›› Issue (2): 219-223.doi: 10.12140/j.issn.1000-7423.2020.02.014

红带锥蝽实验条件下发育特征研究

肖丽贞, 谢汉国^*(), 陈朱云, 欧阳榕, 江典伟

福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州 350001

收稿日期:2019-11-04 出版日期:2020-04-30 发布日期:2020-05-11
通讯作者: 谢汉国
作者简介:肖丽贞（1991-）,女,本科,医师,主要从事寄生虫病防治与媒介防制研究。E-mail： 463250013@qq.com
基金资助:
国家重点研发计划项目(2016YFC1202000);国家重点研发计划项目(2016YFC1202003);国家寄生虫种质资源共享服务平台(平台-TDRC-22)

The developmental characteristics of Triatoma rubrofasciata under experimental conditions

Li-zhen XIAO, Han-guo XIE^*(), Zhu-yun CHEN, Rong OUYANG, Dian-wei JIANG

Fujian Center for Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China

Received:2019-11-04 Online:2020-04-30 Published:2020-05-11
Contact: Han-guo XIE
Supported by:
Supported by the National Key Research and Development Program of China(2016YFC1202000);Supported by the National Key Research and Development Program of China(2016YFC1202003);National Parasite Germplasm Resource Sharing Service Platform(平台-TDRC-22)

摘要/Abstract

摘要：

目的研究红带锥蝽的发育历程,为其调查和防制提供参考依据。方法以福建省漳州市华安县湖坪村采集的红带锥蝽为研究对象,于2018年5月-2019年9月在温度26 ℃、相对湿度60%的实验室环境条件下进行饲养。以ICR小鼠为唯一供血源（供血3 d）,观察并记录红带锥蝽各个发育阶段的孵化、吸血、蜕皮等情况。结果在设定的实验条件下,红带锥蝽可完成整个生活史,包括卵、Ⅰ~Ⅴ龄若虫和成虫等7个阶段,平均历时117.7 d。卵的孵化率为92.2%(391/424)。若虫和成虫均吸血,且每一龄若虫均需饱血才能蜕皮,雌性成虫需吸血才能产卵。Ⅰ~Ⅴ龄若虫的吸血率和蜕皮率分别为86.4%（338/391）和72.4%（283/391）、76.7%（217/283）和58.3%（165/283）、71.5%（118/165）和52.1%（86/165）、91.9%（79/86）和64.0%（55/86）、96.4%（53/55）和56.4%（31/55）。若虫的吸血量随龄期增长而逐渐增大,Ⅰ~Ⅴ龄若虫的平均吸血量分别为（2.9 ± 11.0）、（8.0 ± 3.0）、（14.9 ± 8.8）、（85.4 ± 17.8）、（101.6 ± 54.2）mg,吸血后的平均体质量增重倍数分别为5.4 ± 1.8、3.8 ± 1.2、2.8 ± 1.1、5.1 ± 1.4、2.6 ± 1.1。Ⅴ龄若虫平均吸血量最大,Ⅰ龄若虫吸血后平均体质量增重倍数最大。结论红带锥蝽在实验条件下通过动物饲血能完成生活史,可为相关研究提供实验平台。

关键词: 红带锥蝽, 发育历程, 吸血量, 蜕皮率

Abstract:

Objective To investigate the developmental course of Triatoma rubrofasciata, in order to provide referential basis for its studies and control.Methods T. rubrofasciata were collected from Huping village of Hua’an county of Zhangzhou City, Fujiang Province, and maintained at laboratory conditions at 26℃ with relative humidity of 60%, during May 2018 to September 2019. With ICR mice as the only blood supplier(blood supplied for 3 days), the T. rubrofasciata were observed at various developmental stages on hatching, blood sucking, and molting.Results Under the designed experimental conditions, the T. rubrofasciata could completed the whole life history, including 7 stages covering egg stage, nymph stages Ⅰ-Ⅴ and adult stage, lasting for 117.7 days on average. The hatching rate of eggs was 92.2% (391/424). Both nymphs and adults suck blood, nymphs at all stages could not molt until they had sucked enough amount of blood, and female adults could not produce eggs unless they had suck blood. The blood-taking rate and molting rate of stage Ⅰ-Ⅴ nymphs were 86.4% (338/391) and 72.4% (283/391), 76.7% (217/283) and 58.3% (165/283), 71.5% (118/165) and 52.1% (86/165), 91.9% (79/86) and 64.0% (55/86), 96.4% (53/55) and 56.4% (31/55), respectively. The amount of blood taken by the nymphs increased with the growing stages, being (2.9 ± 11.0), (8.0 ± 3.0), (14.9 ± 8.8), (85.4 ± 17.8), and (101.6 ± 54.2) mg for stageⅠ-Ⅴ nymphs, and the folds of body weight gain after blood-feeding were 5.4 ± 1.8, 3.8 ± 1.2, 2.8 ± 1.1, 5.1 ± 1.4, and 2.6 ± 1.1, respectively. The average amount of blood taken was highest at stage V nymphs, and the fold of body weight gain was highest at stage I nymphs.Conclusion T. rubrofasciata can complete its life cycle by feeding animal blood under the experimental conditions, which provides an laboratory platform for related research.

Key words: Triatoma rubrofasciata, Developmental course, Amount of blood taken, Molting rate

中图分类号:

R384.9

肖丽贞, 谢汉国, 陈朱云, 欧阳榕, 江典伟. 红带锥蝽实验条件下发育特征研究[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(2): 219-223.

Li-zhen XIAO, Han-guo XIE, Zhu-yun CHEN, Rong OUYANG, Dian-wei JIANG. The developmental characteristics of Triatoma rubrofasciata under experimental conditions[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2020, 38(2): 219-223.

图/表 3

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红带锥蝽实验条件下发育特征研究

The developmental characteristics of Triatoma rubrofasciata under experimental conditions

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