关键词: 猪囊尾蚴, 刷状缘结构, 冷冻蚀刻, 超微结构

Abstract: The images of C. cellulosae detected by freeze-itch technique were exactly similar to those observed in the thin section.The scolex microtriches might be divided into two parts, a thick proximal portion and a thinner spinal distal end, the former measuring about 0.2-0.4μm in length, the latter measuring about 1-2μm in length. On the extracellular surface of the proximal portion of microthrix, 1 or 2 particles could occasionally be seen, while numerous particles appeared on the protoplasmic fracture face (PF face) of the proximal portion. The PF face were closely packed and irregularly distributed with membranous particles, which were spherical or subspherical in shape, measured 0.01-0.02μm in diameter. The extracellular fracture face (EF face) of proximal portion and all four membranous faces of spinal end were smooth.The tegumentary plasmic membrane was 0.02μm in thickness, with smooth ES face. The PF face of this membrane packed closely with numerous particles, which were similar with those on the PF face of the microthrix. Numerous globular or discoidal vesicles scattered at random in the tegumentary matrix. Most mitochondria were near the basement membrane of the matrix.In the paranchymal layer, the paranchymal cells, calcareous corpuscles and flame-cells: could easily be seen. Most of the calcareous corpuscles and flame-cells were located closely beneath the muscle layer. The flame-cell of C. cellulosae mainly consisted of three portions,. cell body, a bundle of cilia and a filtered cell lumen. The flame-cell body had a large nucleus and relatively few cytoplasm with several mitochondria. The cilia of flame-cell formed a bunch of cilium tuft bundled up closely by protoplasmic membrane. The diameter of tuft meausred about 2.2-3.0μm. These cilia were arranged regularly in several lines, each line contained 1011- cilia. The total number of the cilia in a flame-cell tuft was 70-90. The cilia appeared as regularly hexagonal near the flame-cell body and cylindrical at the distal end. The cilium was 0.2-0.25μn in diameter and covered by cytoplasmic membrane. On the PF face of the membrane of cilium numerous particles occurred, amounting to 2-10 in number per 0.1 cubimicron area. There was a cone-shaped flame-cell lumen surrounding the central tuft of the cilia. The lumen wall varied greatly. At the head part of lumen, proximal to the flame-cell body, the wall had 1 or 2 rows of stiffening cilia. These cilia were slender than those of the central tuft, arranging in a bar-like structure around the tuft. A leptoslit could usually be found between the cilia. (Figs1-16).