上海市动物园蛇曼氏裂头蚴感染情况

中国寄生虫学与寄生虫病杂志 ›› 2018, Vol. 36 ›› Issue (6): 593-596.

上海市动物园蛇曼氏裂头蚴感染情况

卢艳, 陈家旭, 李浩, 蔡玉春, 艾琳, 储言红, 宋鹏, 陈韶红^*()

中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025

收稿日期:2018-06-20 出版日期:2018-12-30 发布日期:2019-01-08
通讯作者: 陈韶红
基金资助:
上海市卫计委科研课题面上项目（No. 201740076）;国家寄生虫种质资源共享服务平台（No. TDRC-22）;国家重点研发计划项目(No. 2016YFC1202000,No. 2016YFC1202005)

Infection of Spirometra mansoni plerocercoid in snakes from Shanghai Zoo

Yan LU, Jia-xu CHEN, Hao LI, Yu-chun CAI, Lin AI, Yan-hong CHU, Peng SONG, Shao-hong CHEN^*()

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Received:2018-06-20 Online:2018-12-30 Published:2019-01-08
Contact: Shao-hong CHEN
Supported by:
Supported by Surface Project of Shanghai Municipal Commission of Health and Family Planning（No. 201740076）, National Parasite Germplasm Resource Sharing Service Platform（No. TDRC-22）, National Key Research and Development Program of China（No. 2016YFC1202000, No. 2016YFC1202005）

摘要/Abstract

摘要：

目的了解上海市动物园蛇曼氏裂头蚴感染情况。方法 2017年对上海市动物园送检的30条蛇进行解剖检查,分离曼氏裂头蚴,记录寄生部位和数量;新鲜虫体经盐酸卡红染色后,于显微镜下进行形态学鉴定。提取虫体基因组DNA,PCR扩增曼氏裂头蚴线粒体基因细胞色素c氧化酶亚单位1(cytochrome c oxidase subunit 1, cox1)、细胞色素b(cytochrome b, cytb),并进行测序分析。结果上海市动物园送检蛇的裂头蚴总感染率为86.7%（26/30）,共检获裂头蚴626条,感染度为24.1条/蛇。其中6条王锦蛇中有3条感染裂头蚴,感染度为1.33条/蛇;乌梢蛇的感染率为95.8%（23/24）,感染度为27.0条/蛇。裂头蚴主要寄生于蛇肌肉组织,占总数的52.9%（331/626）。显微镜下观察,所获虫体具有曼氏裂头蚴的形态特征。cox1基因和cytb基因的PCR扩增产物分别约为470 bp和1 400 bp。对26条蛇中分离的裂头蚴cox1和cytb基因序列一致性分析结果显示,cox1基因的序列一致性为96.4%~100%,cytb基因的为97.8%~100%。与GenBank中的绦虫序列进行比对,发现其与欧猥迭宫绦虫（GenBank登录号为KP738288、AB374543）的序列一致性为99.8%~100%,与其他种类绦虫的序列一致性为54.2%~84.4%。结论上海市动物园送检蛇的曼氏裂头蚴感染率较高,蛇的曼氏裂头蚴可感染人类,需加强防控。

关键词: 曼氏裂头蚴, 蛇类, 分子鉴定, 动物园

Abstract:

Objective To investigate the infection status of plerocercoid in snakes from Shanghai Zoo in order to provide information for the prevention and treatment of sparganosis. Methods Thirty snakes were provided from Shanghai Zoo and dissected in 2017. The plerocercoids were collected from the snakes, the number of plerocercoids and their location were recorded. The structure of plerocercoid was examined under microscope after being Carmine stained. The total DNA was extracted from the plerocercoids and the DNA fragments for cytochrome c oxidase subunit 1 (cox1) and cytochrome b (cytb) genes were amplified by PCR. The amplified PCR products were sequenced and analyzed. Results The infection rate of plerocercoid in the snakes was 86.7% (26/30) with total 626 plerocercoids found, with an average intensity of infection of 24.1 plerocercoids per snake. The infection rate in snake Elaphe carinata and Zaocys dhumnades was 50.0% (3/6) and 95.8% (23/24), with the average intensity of infection of 1.3 and 27.0 plerocercoids per snake, respectively. Muscular tissue was the most common parasitizing location for the plerocercoids, accounting for 52.9% (331/626). The isolated worms were in accordance with Spirometra mansoni plerocercoids in morphology. The PCR amplified product of cox1 gene was about 470 bp and cytb gene was about 1 400 bp, respectively, sharing 96.4%~100% (cox1) and 97.8%~100% (cytb) sequence identity among the 26 samples. In comparison with the different tapeworm sequences in GenBank, it was found that the amplified cox1 and cytb gene sequences in the plerocercoids were highly homologous to those of S. erinaceieuropaei (99.8%~100%, GenBank Accession No. KP738288, AB374543) and less homologous to other species of tapeworm. Conclusion The snakes collected from Shanghai Zoo are widely infected with S. mansoni plerocercoids, indicating that they can be the infection source of human sparganosis.

Key words: Spirometra mansoni plerocercoid, Snake, Molecular identification, Zoo

中图分类号:

R532.31

卢艳, 陈家旭, 李浩, 蔡玉春, 艾琳, 储言红, 宋鹏, 陈韶红. 上海市动物园蛇曼氏裂头蚴感染情况[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(6): 593-596.

Yan LU, Jia-xu CHEN, Hao LI, Yu-chun CAI, Lin AI, Yan-hong CHU, Peng SONG, Shao-hong CHEN. Infection of Spirometra mansoni plerocercoid in snakes from Shanghai Zoo[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2018, 36(6): 593-596.

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蛇种 Species of snakes	阳性蛇数Positive no. of snake	检出裂头蚴数及寄生部位 Number of the identified pleroceroids and parasitic sites
蛇种 Species of snakes	阳性蛇数Positive no. of snake	肌肉组织Muscle tissue	皮下组织Subcutaneous tissue	体腔 Body cavity
王锦蛇Elaphe carinata	3	2	2	0
乌梢蛇Zaocys dhumnades	23	329	156	137
合计Total	26	331	158	137

虫种 Species	基因序列 Gene sequence	GenBank登录号GenBank accession no.	序列一致性/%Identity/%
欧猥迭宫绦虫Spirometra erinaceieuropaei	cox1	KP738288	99.8
欧猥迭宫绦虫Spirometra erinaceieuropaei	cytb	AB374543	100
阔节双叶槽绦虫Diphyllobothrium latum	cox1	AB511963	84.4
阔节双叶槽绦虫Diphyllobothrium latum	cytb	AB269325	54.2
肥胖带绦虫Taenia saginata	cox1	AB271695	78.5
肥胖带绦虫Taenia saginata	cytb	AB274525	67.0
链状带绦虫Taenia solium	cox1	AB631045	78.2
链状带绦虫Taenia solium	cytb	AY211881	60.2
缩小膜壳绦虫Hymenolepis diminuta	cox1	LC063186	74.7
缩小膜壳绦虫Hymenolepis diminuta	cytb	AF314223	58.9
细粒棘球绦虫Echinococcus granulosus	cox1	KT716270	81.2
细粒棘球绦虫Echinococcus granulosus	cytb	KC709566	65.2
多房棘球绦虫Echinococcus multilocularis	cox1	AB385610	61.9
多房棘球绦虫Echinococcus multilocularis	cytb	AB374426	66.7