关键词: 日本血吸虫病, 改良加藤厚涂片法, 尼龙绢集卵孵化法, 胶体染料试纸条法（DDIA, F-ELISA, 间接红细胞凝集试验（IHA）

Abstract: 【Abstract】 Objective To evaluate the effectiveness of routinely used assays for schistosomiasis diagnosis in the field. Methods From late November to early December 2005， 6-65 years old inhabitants from 3 endemic villages were examined by Kato-Katz technique （3 thick smears） and nylon bag sedimentation/hatching method. At the same time， dipstick dye immunoassay（DDIA）， fast enzyme linked immunosorbent assay （F-ELISA）， indirect haemagglutination test A （IHA-A） and B （ IHA-B） were carried out in parallel. Results 1 864 people were examined by stool examination with an average positive rate of 9.7%. The missing rate of DDIA was relatively stable in medium and heavily endemic areas of schistoso-miasis. The missing rate of nylon bag sedimentation/hatching method was 25% and relatively stable when the number of eggs per gram of feces（EPG） was larger than 100. The average positive rate of DDIA， F-ELISA， IHA-A and IHA-B was 47.8%， 50.0%， 66.3% and 40.1% respectively. Using stool examination as the gold standard， the sensitivity of DDIA， F-ELISA， IHA-A and IHA-B was 75.3％， 65.8％， 85.6％ and 76.0%; and the specificity was 55.1％， 51.7％， 35.7% and 63.6％， respectively. Among the four sero-diagnostics， the specificity， Youden index， positive likelihood rate and coincidence of IHA-B were the highest. Conclusion Kato-Katz method is more stable and effective than nylon bag sedimentation/hatching method in medium and heavily endemic areas of schistosomiasis japonica. The sensitivity and spec-ificity of these four diagnosis kits are lower than 90%.

Key words: Schistosomiasis japonica, Kato-Katz technique, Hatching, DDIA, F-ELISA, IHA