中国寄生虫学与寄生虫病杂志 ›› 1994, Vol. 12 ›› Issue (2): 100-103.

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湖北钉螺血淋巴细胞的形态及其吞噬功能

王晓勤,裘丽姝,何毅勋,毛守白   

  1. 中国预防医学科学院寄生虫病研究所
  • 收稿日期:2017-01-06 修回日期:2017-01-06 出版日期:1994-05-31 发布日期:2017-01-06

MORPHOLOGY AND PHAGOCYTIC ACTIVITY OF HEMOCYTES OF ONCOMELANIA HUPENSIS

  • Received:2017-01-06 Revised:2017-01-06 Online:1994-05-31 Published:2017-01-06

摘要: 作者对日本血吸虫中间宿主湖北钉螺的血淋巴细胞的形态及体外行为进行了观察。钉螺血淋巴液中常见的细胞为伸展性阿米巴细胞。来自安徽及云南的钉螺的此种细胞大小分别为8.42±0.73×7.46±0.52μm及7.63±0.65×6.85±0.54μm。细胞核呈圆形,胞浆内有嗜酸性颗粒,细胞接触玻片后,伸出伪足。两地螺的阿米巴细胞均具有体外吞噬羊红细胞的能力。本文介绍了采集湖北钉螺血淋巴液的方法,并讨论了该方法在研究寄生虫-宿主关系及在日本血吸虫病流行病学研究等方面的意义。

关键词: 湖北钉螺, 血淋巴细胞

Abstract: Hemocytes from Oncomelania hupensis hemolymph were studied in vitro. Spreading amoebocytes were the most common cell type.The average sizes of amoebocytes from Anhui and Yunnan were 8.42±0.73×7.46±0.52 μm and 7.63±0.65×6.85±0.54 μm, respectively. The nucleus was round in shape,and eosinophile granular inclusions were found in the endocytoplasm.Contacting with slide,the cells became flattened with extending pseudopodia. The ability of amoebocytes from snails of both localities to phagocytose sheep red blood cells was confirmed in vitro. The method to collect hemolymph from Oncomelania hupensis was developed, and the promise of this method in studying host parasite relationship as well as epidemiological investigation of schistosomiasis japonica in the mainland of China was discussed.

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