中国寄生虫学与寄生虫病杂志 ›› 1993, Vol. 11 ›› Issue (4): 286-287.

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间接荧光抗体试验检测感染广州血管圆线虫大鼠血清抗体的观察

梁浩昆,晋雪香,冯祖梅,王梅英,潘松涛,苏寿(氵氐)   

  1. 广州医学院寄生虫学教研室; 河南医科大学寄生虫学教研室; 河南医科大学寄生虫学教研室; 河南医科大学寄生虫学教研室; 广州医学院寄生虫学教研室; 河南医科大学寄生虫学教研室
  • 收稿日期:2017-01-06 修回日期:2017-01-06 出版日期:1993-11-30 发布日期:2017-01-06

DETECTION OF SERUM ANTIBODIES IN RATS INFECTED WITH ANGIOSTRONGYLUS CANTONENSIS BY INDIRECT FLUORESCENT ANTIBODY TEST

  • Received:2017-01-06 Revised:2017-01-06 Online:1993-11-30 Published:2017-01-06

摘要: 用广州血管圆线虫成虫切制成 4 μm厚的冰冻切片作抗原,用间接荧光抗体试验检测感染广州血管圆线虫大鼠血清的抗体,结果在感染后 2 wk的 50份鼠血清中,呈阳性反应46份,阳性率为 92.0%;在感染后 4 wk的 19份鼠血清中,呈阳性反应 19份,阳性率为 100%;对照的阴性血清及盐水均呈阴性反应。本法检出率高,且感染早期出现阳性反应,可作广州血管圆线虫病早期辅助诊断。

关键词: 广州血管圆线虫, 间接荧光抗体试验

Abstract: Frozen section specimens with a thickness of 4 μm were made from adult worms of An-giostrongylus cantonensis in order to detect the serum antibodies in rats infected with An-giostrongylus cantonensis by indirect fluorescent antibody test (IFAT). The results showed that the positive rates of the serum samples 2 and 4 weeks after infection were 92. 0%(46/ 50) and l00% (19/19), respectively. The results of serum samples in healthy rats and normal saline (as control) were negative. This technique proved to have a highly detectable rate and the positive reaction might occur in early infection. It might be used for the early supplementary diagnosis of angiostrongyliasis.