中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (2): 122-125.

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应用特异单克隆抗体测定猪囊尾蚴病人血清和脑脊液中的循环抗原

陈捷平,张夏英,谭巍,刘明方,刘国玲,胡永秀   

  1. 中日友好医院临床医学研究所免疫研究室; 北京热带医学研究所蠕虫病室; 北京热带医学研究所蠕虫病室; 中日友好医院临床医学研究所免疫研究室; 中日友好医院临床医学研究所免疫研究室; 北京热带医学研究所蠕虫病室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1991-05-31 发布日期:2017-01-09

DETERMINATION OF CIRCULATING ANTIGEN IN CYSTICERCOSIS PATIENTS USING MCAB-BASED ELISA

  • Received:2017-01-09 Revised:2017-01-09 Online:1991-05-31 Published:2017-01-09

摘要: 我们应用特异的抗猪囊尾蚴抗原的单克隆抗体(CCy1),建立了单克隆抗体抑制性ELISA方法以测定囊尾蚴病患者的循环抗原(CAg)。测定灵敏度为ng水平。83例囊尾蚴病患者血清的CAg阳性率为71.1%。其范围为0.16-128μg/ml。对41例囊尾蚴病患者,同时测定了血清和脑脊液中的CAg。单项或两项阳性的总阳性率为90.2%。114例正常人血清、107例其它寄生虫病患者(包括30例包虫病患者)的血清及10例非寄生虫病患者的脑脊液的CAg量均为零。有23例囊尾蚴病患者治疗半年到1年后,有21例抗原浓度为零。另2例分别为0.64μg/ml和1.6μg/ml。表明测定CAg不仅可以确定活动性囊尾蚴感染,而且可考核疗效。

关键词: 猪囊尾蚴, 猪囊尾蚴病, 循环抗原, 单克隆抗体, ELISA

Abstract: For the first time, the determination of circulating antigen in patients with cysticetocsis using specific monoclonal antibody against cysticercus antigen (CCy1) based on inhibitive ELISA was reported. The circulating antigens were detected in the sera from 83 patients with cysticercosis, the positive rate being 71.1%. The range of the detectable serum antigen concentrations was 0.16-128μg/ml. In 41 cases of cysticercosis patients, circulating antigens were determined in both sera and cerebrospinal fluids, the positive rates being 68.3% and 78% respectively, the total positive rate being 90.2%. (Fig.l) In 114 sera from normal persons, circulating antigen was absent. In 30 sera from patients with hydatidosis, 30 with clonor-chiasis, 20 with schistosomiasis japonica, 24 with paragonimiasis, and 5 sera collected from non-parasitosis patients, serum circulating antigen was not detectable. In another 10 non parasitosis patients, circulating antigen was not found in cerebrospinal fluid. After chemotherapy for one half to one year, in 21 out of 23 cases of cysticercosis patients, the circulating antigen levels in sera dropped to zero, except for two cases having an antigen evel of 0.64μg/ml and 1.6μg/ml, respectively. Our results showed that the determination of the circulating cysticercus antigen was very specific for the diagnosis of active infection of cysticercosis and could be used as a rational tool in monitoring the effectiveness of chemotherapy.