山东荣成八代按蚊分类地位的探讨

摘要目的探讨山东荣成八代按蚊的分类地位。方法于2009年8月底在山东荣成西郊王家村采集饱血八代按蚊，饲养得子代，对子代成蚊进行形态学鉴定。抽提各型成蚊足的DNA，PCR扩增rDNA?鄄ITS2片段后测序，并用DNAstar7.1软件对所获荣成八代按蚊的rDNA?鄄ITS2序列与文献记录的山东荣成八代按蚊（YSD，AY306128）、四川省八代按蚊（YSC，AY170925）、辽宁省八代按蚊（YLN，AY170923）和韩国八代按蚊（YK，AF146749）的相关序列进行多重比对。结果共采集饱血八代按蚊56只，其中7只顺利产卵，饲养共获得子代成蚊354只，包括八代按蚊型（Y型，成蚊翅脉端白斑和V5.2顶繸白斑均有）240只、近黑按蚊型（P型，两者均无）8只和杂合型（H型，两者其一）106只。亲本和子代成蚊各型间rDNA-ITS2段序列（JN865249，JN865246，JN865247，JN865248）同源性为98.7~100%，与四川省八代按蚊、辽宁省八代按蚊和韩国八代按蚊的rDNA-ITS2段序列同源性分别为98.5%~99.3%、98.7%~99.6%和98.7%~100%，而与原山东荣成八代按蚊同源性仅为67.1%~68.5%。结论山东荣成的八代安蚊形态具有Y型、P型和H型等不同翅斑形态表型，但其rDNA-ITS2序列特征均与近黑按蚊近缘，可推断当地有近黑按蚊分布。

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关键词：荣成八代按蚊近黑按蚊分类地位

Abstract： Objective To clarify the taxonomic status of Anopheles yatsushiroensis in Rongcheng City of Shandong Province. Methods By the end of August 2009，Anopheles yatsushiroensis were collected in the western suburbs of Wangjia Village of Rongcheng City，and raised for the next generation of various types of adult mosquitoes. Through morphological identification of filial generation various types, mosquito feet of 1-2 mosquitoes of various types were taken for complete DNA extraction，and complete sequence analysis of rDNA-ITS2 alignment was made by PCR. Multiple sequnence comparison was carried out with those previously documented by DNAstar7.1，including An. yatsushiroensis in Shandong Province（YSD，AY306128），An. yatsushiroensis in Sichuan Province（YSC，AY170925），An. yatsushiroensis in Liaoning Province（YLN，AY170923） and An. yatsushiroensis from South Korea （YK，AF146749）. Results 56 An. yatsushiroensis with blood meal were collected in Rongcheng City，of which 7 successfully laid eggs, and received 354 adult mosquitoes of filiar generation，including 240 An. yatsushiroensis （Y type），8 An. pullus （P type） and 106 hybrid type（H）. The rDNA-ITS2 sequence alignment homology between single-parent female offspring of adult mosquitoes and various types of An. yatsushiroensis segments （JN865249，JN865246，JN865247，JN865248）was 98.7-100%，and the rDNA-ITS2 sequence alignment homology to those from Sichuan（YSC），Liaoning （YLN） and Korea （YK） was 98.5%-99.3%，98.7%-99.6% and 98.7 to 100%，respectively, while to the original An. yatsushiroensis from Rongcheng（YSD）, it was only 67.1%-68.5%. Conclusion There are Y，P and H types of An. yatsushiroensis in Rongcheng City，Shandong Province，but the rDNA-ITS2 sequence is close to An. pullus. The local distribution of An. pullus is deduced.

Keywords： Rongcheng Anopheles yatsushiroensis Anopheles pullus Taxonomy

引用本文:

周晓俊, 施文琦, 张仪, 岳爱萍.山东荣成八代按蚊分类地位的探讨[J] 中国寄生虫学与寄生虫病杂志, 2011,V29(6): 443-446

ZHOU Xiao-Dun, SHI Wen-Qi, ZHANG Yi, YUE Ai-Ping.Taxonomic Status of Anopheles yatsushiroensis in Rongcheng City of Shandong Province[J] , 2011,V29(6):443-446