›› 2011, Vol. 29 ›› Issue (4): 19-271-273.

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The Target of Musca domestica Cecropin on Human Hepatocelluar Carcinoma BEL-7402 Cells

 JIN  Xiao-Bao,   Li-Xiao-Bo,   Zhu-Jia-Yong,   Lu-Xue-Mei,  Shen-Juan,   Chu-Fu-Jiang,   Mei-Han-Fang   

  1. School of Basic Courses,Guangdong Pharmaceutical University,Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances,Guangdong Pharmaceutical University,Guangzhou 510006,China
  • Online:2011-08-30 Published:2012-09-27

Abstract: Human hepatocelluar carcinoma BEL-7402 cells were treated with 50 μmol/L Musca domestica cecropin for 12 h, and observed under scanning electron microscope. The effect of Musca domestica cecropin labeled with FITC (FITC-cecropin) on BEL-7402 cells was detected by laser scanning confocal microscopy. The scanning electron microscopy showed that most microvilli on the surface of BEL-7402 cells disappeared at 12 h after cecropin treatment. The laser scanning confocal microscopy revealed that most FITC-cecropin combined with BEL-7402 cell membrane, and partly in the cytoplasm.

Key words: Scanning electron microscope;Laser scanning confocal microscope;Cecropin;Tumor cell;
                 ,
Membrane