CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2022, Vol. 40 ›› Issue (2): 181-186.doi: 10.12140/j.issn.1000-7423.2022.02.008

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Characteristics of alveolar echinococcosis cases and genetic polymorphism of the parasite from Xinyuan County, Xinjiang

CAI Ren1(), REN Yuan2, MI Rong-sheng3, GUO Gang1, QI Wen-jing2, ZHANG Zhuang-zhi4, GUO Bao-ping1,*()   

  1. 1. Institute of Clinical Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
    2. State Key Laboratory of Pathogenesis, Prevention and Treatment of Central Asian High Incidence Diseases, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
    3. Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
    4. Veterinary Research Institute, Xinjiang Academy of Animal Sciences (Animal Clinical Medical Research Center of Veterinary Research Institute, Xinjiang), Urumqi 830000, China
  • Received:2021-08-12 Revised:2021-10-01 Online:2022-04-30 Published:2022-04-07
  • Contact: GUO Bao-ping E-mail:181132786@qq.com;guobaopingfeng@126.com
  • Supported by:
    Natural Science Foundation of Xinjiang Uygur Autonomous Region(2019D01C315)

Abstract:

Objective To understand the characteristics of alveolar echinococcosis (AE) cases and the genetic polymorphism of the parasites in Xinyuan County, Xinjiang, to provide reference information for formulating developing prevention and control strategies for AE. Methods During 2016—2020, information on AE patients and some surgically resected liver lesion samples from Xinyuan County in the biological sample bank of the First Affiliated Hospital of Xinjiang Medical University were collected. Genomic DNA of the liver lesion samples was extracted, and the mitochondrial NADH dehydrogenase 2 (nad2) gene was amplified by PCR and sequenced. The PCR products were sequenced and spliced for Blast comparison. Mega10.0 software was used to construct a phylogenetic tree by the neighbor-joining method, DnaSPv5 software was used to analyze the genotype of amplified sequence, and Network10.0 software was used to make nad2 genotype network map. Results A total of 28 AE cases were collected, including 7 in rural areas and 21 in Kunes River Basin, among which 60.71% (17/28) were male, and 39.29% (11/28) were female (χ2 = 0.704, P < 0.05). Ethnic distribution was mainly Han (42.86%, 12/28) and Kazak (39.29%, 11/28), and the highest infection rate among Mongolians was 11.17%. There was a significant difference in the infection rate among different ethnic groups (χ2 = 61.08, P < 0.01). Farmers and herdsmen were the dominant occupations, accounting for 92.86% (26/28), while only 7.14% (2/28) were of other occupations. There was no significant difference in infection rate among different age groups (χ2 = 0.418, P > 0.05). A expected, 25 samples were amplified by PCR with a fragment size of 1 031 bp. Sequence alignment results showed that there were four genotypes: H1, H2, H3 and H4, among which H1 was the main genotype and the sequence consistency was 100% with Kazakhstan (GenBank accession no. AB461406). Phylogenetic tree results showed that the four genotypes in Xinyuan county isolates were in the same branch as the Poland (GenBank accession no. KY205700) and Kazakhstan (GenBank accession no. AB461406), but more distantly related to France (GenBank Accession no. AB461404), Austria (GenBank accession no. AB461403), Canada (GenBank accession no. JF751036), Alaska (GenBank accession no MT429275), Indiana, USA (GenBank accession no. AB461409) and Inner Mongolia, China (GenBank accession no. AB461411). According to the results of centering on the H1, the genotype network diagram showed H2, H3, H4, Sichuan of China, Poland, Canada and Japan were scattered distributed which indicated that the isolates with H1 has a very close relationship, but it is not in the same clade with France, Austria, Canada, Alaska, USA isolates, and had a distant relationship. Conclusion The majority of AE patients were Han and Kazakian farmers and herdsmen, and the Mongolians had the highest infection rate. There were 4 genotypes in AE patients, among which H1 was the main genotype in Xinyuan County.

Key words: Alveolar echinococcoisis, Xinyuan County, Nad2 gene, Haplotype

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