CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2020, Vol. 38 ›› Issue (3): 279-285.doi: 10.12140/j.issn.1000-7423.2020.03.004
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TAN Xiao*(), XIAO Chu-li, XIAO Fei, WANG Shuo, QING Rui, HUANG Ze-zhi
Received:
2019-12-26
Online:
2020-06-30
Published:
2020-07-07
Contact:
Xiao TAN
E-mail:syjjbd@126.com
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TAN Xiao, XIAO Chu-li, XIAO Fei, WANG Shuo, QING Rui, HUANG Ze-zhi. IL-18 enhances the immunoprotective effect of pcDNA3.1/SjOST48 against Schistosoma japonicum infection[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(3): 279-285.
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Table 1
Worm and egg reduction rates of immunized mice (n = 15)
分组 Group | 成虫数 Worm burden | 减虫率 Worm reduction rate/% | 虫卵数/×104 Average number of eggs per gram liver tissue/×104 | 减卵率 Egg reduction rate/% |
---|---|---|---|---|
PBS | 26.63 ± 3.41 | 36.86 ± 6.25 | ||
pcDNA3.1 | 26.32 ± 2.34 | 35.25 ± 4.21 | ||
pcDNA3.1/IL-18 | 23.11 ± 1.76 | 13.22a | 30.71 ± 3.91 | 16.68a |
pcDNA3.1/SjOST48 | 18.06 ± 1.21 | 32.18a | 23.67 ± 2.54 | 35.78a |
pcDNA3.1/IL-18+pcDNA3.1/SjOST48 | 14.33 ± 1.08 | 46.19 | 20.39 ± 2.31 | 44.68 |
Fig. 5
Pathological changes of liver in each group (HE staining, × 40) A, B: In the PBS and pcDNA3.1 groups, hepatocyte degeneration and necrosis were observed, the worm egg granulomas had a larger size (marked in red), inflammatory infiltration was evident, and a small number of eosinophils and lymphocytes was seen. C: The granulomas in the pcDNA3.11/ IL-18 group were decreased and smaller compared to the PBS group and the pcDNA3.1 group. D, E: In the pcDNA3.1/SjOST48 and pcDNA3.1/IL-18+pcDNA3.1/SjOST48 groups, the granulomas were greatly reduced, accompanied by milder inflammatory infiltration.
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