关键词: 牛带绦虫, 诊断, 线粒体细胞色素c氧化酶1基因

Abstract:

Epidemiological information on the first case of taenia infection in Ninghai County was collected and analyzed. Eggs in the patient feces were founded using saline smear method. The patient was dewormed with areca and pumpkin seeds and the expelled worm was collected for morphological identification. The cytochrome c oxidase 1(cox1) gene was amplified from the worm using PCR. The epidemiological results showed that the patient had no history of travelling out of the living area, but once experienced eating undercooked beef in a hot pot in September, 2017. The patient started to have manifestation of diarrheal and hunger on November 10, 2017. A white, flat and noodle-like segment of worm with 30 cm in length was first observed in the feces 2-weeks later. One more segment with 2 cm long was then seen in feces. The patient went to see a doctor and was diagnosed as taeniasis. The eggs were observed in the fecal sample with an oncosphere inside without membrane. The expelled worm was total 5.32 m in length, with a slim and long anterior part and gradually wide body connected with segments. The scolex was not observed. The gravid proglottids contained 24-28 uterus branches on each side. The morphological examination showed consistency with that of Taenia saginata. A DNA fragment with 832 bp of cox1 were amplified by PCR and the sequence results showed 99%, 96% and 88% identity to cox1 of Taenia saginata, Taenia asiatica and Taenia solium respectively (GenBank accession number: AB107239.1, AB107235.1, AB066485.1). The results confirm that this is a case of Taenia saginata infection.

Key words: Taenia saginata, Diagnosis, Mitochondrial cytochrome c oxidase 1 gene