CRISPR/Cas9系统在寄生虫领域的研究进展

摘要/Abstract

摘要：

【提要】 CRISPR/Cas9是一种有效的基因组靶向修饰系统,能够实现对基因组特定位点的基因敲除、定点插入和基因修复等遗传操作,为寄生虫的基因功能分析、药物靶点和疫苗分子筛选等研究提供了技术创新平台。本文对CRISPR/Cas9系统的原理及其在刚地弓形虫（Toxoplasma gondii）、疟原虫（Plasmodium）、锥虫（Trypanosoma）和利什曼原虫（Leishmania）等重要寄生虫研究中的新进展进行综述,分析讨论现阶段该系统在寄生虫研究应用方面存在的问题和优化策略。

关键词: CRISPR/Cas9系统, 基因编辑, 寄生虫

Abstract:

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats)/(CRISPR-associated protein 9) is an efficient genome editing system for targeted gene disruption, site-specific insertion of foreign DNA, gene repair, etc., which provides an innovation platform of technology for functional analysis of parasite genes and selection of drug targets and vaccine candidates. Here, we review the mechanism of CRISPR/Cas9 and some recent findings on the use of CRISPR/Cas9 in parasite research, particularly in studies of Toxoplasma gondii, Plasmodium, Trypanosoma and Leishmania. The challenges and optimization strategies of the CRISPR/Cas9 system at the present stage are also discussed.

Key words: CRISPR/Cas9 system, Gene editing, Parasite

中图分类号:

付益修, 孔庆明, 陆绍红. CRISPR/Cas9系统在寄生虫领域的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(3): 299-304.

Yi-xiu FU, Qing-ming KONG, Shao-hong LU. Research development of CRISPR/Cas9 system on parasitic studies[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2017, 35(3): 299-304.

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