上海某动物园灵长类动物隐孢子虫感染情况

中国寄生虫学与寄生虫病杂志 ›› 2017, Vol. 35 ›› Issue (2): 136-139.

上海某动物园灵长类动物隐孢子虫感染情况

张尚瑞¹, 沈玉娟², 王斌¹, 刘华², 曹胜魁², 袁忠英², 曹建平², 刘晖^1,^*()

1 遵义医学院寄生虫学教研室,遵义 563003
2中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025

收稿日期:2016-11-21 出版日期:2017-04-20 发布日期:2017-05-02
通讯作者: 刘晖
基金资助:
国家公益性卫生行业科研专项（No. 201502021）;上海市第四轮三年行动计划（No. 15GWZK01）;中国疾病预防控制中心青年科研基金（No. 2016A102）

Investigation of Crytosporidium infection in primates in a zoo of Shanghai

Shang-rui ZHANG¹, Yu-juan SHEN², Bin WANG¹, Hua LIU², Sheng-kui CAO, Zhong-ying YUAN², Jian-ping CAO², Hui LIU^1,^*()

1 Department of Parasitology, Zunyi Medical College, Zunyi 563003, China
2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Received:2016-11-21 Online:2017-04-20 Published:2017-05-02
Contact: Hui LIU
Supported by:
Supported by the Chinese Special Program for Scientific Research of Public Health(No. 201502021), the Fourth Round of Three-Year Public Health Action Plan of Shanghai, China(No. 15GWZK01), and a Project Sponsored by the Young Scholar Scientific Research Foundation of China CDC(No. 2016A102)

摘要/Abstract

摘要：

目的了解上海地区某动物园灵长类动物隐孢子虫感染情况。方法 2013年3月至2014年10月,采集上海市某动物园灵长类动物的新鲜粪样,经金胺酚-改良抗酸染色后,镜下观察隐孢子虫卵囊。采用巢式PCR扩增18S rRNA基因,测序分析后鉴定虫种,采用Mega7软件用邻接法构建系统进化树。结果共采集8科37种灵长类动物新鲜粪样83份,镜检未见隐孢子虫卵囊。巢式PCR检测其中3份粪样（6号红猩猩、42号和44号山魈的粪样）隐孢子虫阳性,DNA阳性率为3.61%（3/83）。测序结果显示,与安氏隐孢子虫（Cryptosporidium andersoni）（GenBank登录号KT175424、KF271479、KF271467）的同源性均为99%。系统进化树显示,3份阳性样品的基因序列与其他来源的安氏隐孢子虫（GenBank登录号KT175424、KF271479、KF271467、KF826314、KJ917578）亲缘关系相近,处于同一分支,表明本次分离的虫株为安氏隐孢子虫。结论上海地区某动物园灵长类动物存在安氏隐孢子虫感染,具有潜在人兽共患的风险。

关键词: 隐孢子虫, 动物园, 灵长类动物, 基因型, 感染

Abstract:

Objective To determine Cryptospordium infection in primates in a zoo of Shanghai. Methods Fresh fecal samples of primates were collected in a zoo in Shanghai from March 2013 to October 2014. The samples underwent Auramine phenol and modified acid-fast staining to detect oocysts of Cryptosporidium. The 18S rRNA gene was amplified by nested PCR. The PCR products were sequenced and phylogenetic trees were constructed with the neighbor-joining method using Mega 7 software. Results A total of 83 fecal samples were collected from primates from 8 families, 37 species. None was positive for oocysts of Cryptospordium. Nested PCR of 18S rRNA gene generated positive products from 3 samples from No. 3 orangutan, No. 42 and No. 44 mandrills, respectively, with a positive rate of 3.61%(3/83). The sequences of the three products all shared 99% identity with Cryptosporidium andersoni（GenBank Accession No. KT175424, KF271479 and KF271467). The phylogenetic tree showed that the three samples were in the same branch as other species of Cryptosporidium andersoni(GenBank Accession No. KT175424, KF271479, KF271467, KF826314 and KJ917578). This implied that the three Cryptosporidium isolates were C. andersoni. Conclusion Cryptospordium infection exists in primates in the zoo in Shanghai, indicating a potential risk of zoonosis.

Key words: Cryptosporidium, Zoo, Primate, Genotype, Infection

中图分类号:

R382.3

张尚瑞, 沈玉娟, 王斌, 刘华, 曹胜魁, 袁忠英, 曹建平, 刘晖. 上海某动物园灵长类动物隐孢子虫感染情况[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(2): 136-139.

Shang-rui ZHANG, Yu-juan SHEN, Bin WANG, Hua LIU, Sheng-kui CAO, Zhong-ying YUAN, Jian-ping CAO, Hui LIU. Investigation of Crytosporidium infection in primates in a zoo of Shanghai[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2017, 35(2): 136-139.

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