中国寄生虫学与寄生虫病杂志

• 研究简报 • 上一篇    下一篇

河南省HIV携带者刚地弓形虫感染情况

白晨倩1,王东1,姚志军1,王帅1,张露文1,刁风金1,任笑盈1,何婵婵1,王兆红1,马金超1,王苏康1,彭帮淦1,胡翠1,安戈2,刘世国1 *   

  1. 1 新乡医学院基础医学院人体寄生虫学教研室,新乡453000;2 郑州市疾病预防控制中心,郑州 450000
  • 出版日期:2016-02-28 发布日期:2016-03-11

Toxoplasma gondii Infection in HIV-infected Individuals in Henan Province

BAI Chen-qian1,WANG Dong1,YAO Zhi-jun1,WANG Shuai1,ZHANG Lu-wen1,DIAO Feng-jin1,REN Xiao-ying1,HE Chan-chan1,WANG Zhao-hong1,MA Jin-chao1,WANG Su-kang1,PENG Bang-gan1,HU Cui1,AN Ge2,LIU Shi-guo1 *   

  1. 1 Department of Human Parasitology, School of Basic Medical Science, Xinxiang Medical University, Xinxiang 453000, China; 2 Zhengzhou Center for Disease Control and Prevention, Zhengzhou 450000, China
  • Online:2016-02-28 Published:2016-03-11

摘要:

收集河南省1 038份HIV携带者外周血样,提取其基因组DNA后,利用巢式PCR技术扩增弓形虫529 bp重复序列基因,凝胶电泳检测目的片段。1 038份HIV携带者全血血样中(男762份,女276份),弓形虫PCR阳性血样66份,弓形虫PCR阳性率为6.4%(66/1 038)。男性和女性的弓形虫PCR阳性率分别为6.3%(48/762)和6.5%(18/276)。已婚508份,未婚、离异或丧偶530份,其弓形虫PCR阳性率分别为4.9%(25/508)和7.7%(41/530)(χ2=3.451,P>0.05)。高中及以上学历、高中以下学历人群的弓形虫PCR阳性率分别为6.9%(34/489)和5.8%(32/549)(χ2=0.545,P>0.05)。20~40岁组人群的弓形虫PCR阳性率最高,为7.6%(31/410)。有性病史、无性病史和病史不详者的弓形虫PCR阳性率分别为8.0%(9/113)、6.5%(50/773)和4.6%(7/152)(χ2=0.355,P>0.05)。

关键词: 艾滋病病毒, 刚地弓形虫, 巢式PCR, 河南

Abstract:

 Genomic DNA was extracted from 1 038 peripheral blood samples from HIV-infected individuals in Henan Province. One-step single-tube nested PCR was performed to amplify the 529 bp repeating sequences of Toxoplasma gondii. Of the 1 038 samples (762 from males and 276 from females), 66 showed positive PCR results, with a positive rate of 6.4%. The PCR positive rate in males and females was 6.3% (48/762) and 6.5% (18/276) respectively. The PCR positive rate in the married HIV individuals was 4.9%(25/508), and that in unmarried, divorced and widowed HIV individuals was 7.7% (41/530)(χ2 = 3.451, P> 0.05). The PCR positive rate in HIV individuals with a high-school educational level or above was 6.9%(34/489), and that in those below the high-school level was 5.8% (32/549)(χ2 = 0.545, P> 0.05). The highest infection rate was in the age group of 20-40 years(7.6%, 31/410). In addition, the Toxoplasma infection rate in those with and without a history of venereal diseases, and those with an unknown history was 8.0%(9/113), 6.5%(50/773) and 4.6%(7/152) respectively (χ2 = 0.355, P> 0.05).

Key words: Human immunodeficiency virus, Toxoplasma gondii, Nested PCR, Henan Province