中国寄生虫学与寄生虫病杂志

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重组酶聚合酶扩增技术及其在寄生虫检测中的应用

郑文斌1,2,吴耀东1,马剑钢1,2,朱兴全1,周东辉1 *   

  1. 1中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,兰州 730046;2吉林农业大学动物科学技术学院,长春 130118
  • 出版日期:2015-10-30 发布日期:2016-01-06

Recombinase Polymerase Amplification and its Applications in Parasite Detection

ZHENG Wen-bin1,2, WU Yao-dong1, MA Jian-gang1,2, ZHU Xing-quan1, ZHOU Dong-hui1 *   

  1. 1 State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China; 2 College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
  • Online:2015-10-30 Published:2016-01-06

摘要:

重组酶聚合酶扩增技术(RPA)是以T4噬菌体体内核酸复制机制为原理,以多种蛋白及酶参与的一种新型核酸等温扩增技术,可在37~42 ℃条件下,20 min内将核酸扩增至可检测水平。具有较高的敏感性和特异性,且操作简单,可在非实验室条件下实现对核酸的快速检测。自2006年问世后,已广泛应用于农业、食品安全、医学和转基因检测等多个领域。本文综述了RPA技术的研究进展及其在寄生虫检测中的应用。

关键词: 重组酶聚合酶扩增技术(RPA), 寄生虫, 隐孢子虫, 贾第鞭毛虫, 疟原虫

Abstract:

Recombinase polymerase amplification(RPA) is a recently-developed isothermal nucleic-acid-amplification technology that is based on the nucleic acid replication mechanism in T4 bacteriophage. With this technique, nucleic-acid templates can be amplified to measurable levels within 20 min at 37-42 ℃. The RPA process has high sensitivity and specificity, and is simple to operate,  thus nucleic acids can be detected rapidly in non-laboratory conditions. Since its development in 2006, the RPA technique has been applied in agriculture, food safety, medicine, transgene detection, etc. In this review, we will give an overview on the research progress of RPA and its application in parasite detection.