中国寄生虫学与寄生虫病杂志

• 研究简报 • 上一篇    下一篇

弓形虫TgROP21基因克隆及生物信息学分析

史世俊,崔勇,李瑾,王洪法,尹昆,魏庆宽,黄炳成,孙慧,刘功振*   

  1. 山东省医学科学院,山东省寄生虫病防治研究所,济宁 272033
  • 出版日期:2015-08-30 发布日期:2015-09-10

Cloning and Bioinformatics Analysis of Toxoplasma gondii ROP21 Gene

SHI Shi-jun,CUI Yong,LI Jin,WANG Hong-fa,YIN Kun,WEI Qing-kuan,HUANG Bing-cheng,SUN Hui,LIU Gong-zhen*   

  1. Shandong Academy of Medical Sciences, Shandong Institue of Parasitic Diseases, Jining 272033, China
  • Online:2015-08-30 Published:2015-09-10

摘要:

PCR扩增弓形虫棒状体蛋白21(TgROP21)全长基因序列,利用SignaIP和TMHMM在线网站预测分析其信号肽、跨膜区;DNAStar软件分析其亲水性、抗原指数;联合运用ExPASY和PRODATA在线网站对TgROP21的功能域及三级结构进行建模。PCR产物可见1条2 022 bp左右的片段,与预期片段大小相同。成功预测出TgROP21的信号肽、跨膜区、亲水性、抗原指数、功能域和三级结构。

关键词: 弓形虫, 弓形虫棒状体蛋白21, 生物信息学

Abstract:

The full-length gene sequence of Toxoplasma gondii ROP21(TgROP21) gene was amplified with PCR. The signaling peptide and transmembrane domain of TgROP21 protein were predicted by SignaIP and TMHMM online predictive sites, and the hydrophilicity and antigenic index of this protein were ananlyzed with DNAStar software. Meanwhile, the functional domains and tertiary structure were modeled by combined use of ExPASY and PRODATA online sites. As expected, the PCR results revealed one band at 2 022 bp. The signaling peptide, transmembrane domain, hydrophilicity, antigen index, functional domain and 3D structure of TgROP21 were successfully predicted. This work may provide a theoretical foundation for further verification of TgROP21 function. 

Key words: Toxoplasma gondii, Rhoptry protein 21, Bioinformatics