Th17细胞及其细胞因子白细胞介素-17在粉尘螨致敏哮喘小鼠中的变化

中国寄生虫学与寄生虫病杂志

Th17细胞及其细胞因子白细胞介素-17在粉尘螨致敏哮喘小鼠中的变化

吕勤1，杨小猛2，肖小军2，陈思2，杨平常2，刘志刚2，张敏1*

1 深圳大学第一附属医院呼吸内科，深圳 518037；2 深圳大学过敏反应和免疫学研究所，深圳 518060

出版日期:2015-04-30 发布日期:2015-05-04

Percentage of Th17 Cells in Spleen and IL-17 Level in Bronchoalveolar Lavage Fluid in Dermatophagoides farinae Allergic Asthma Mice

LV Qin1，YANG Xiao-meng2，XIAO Xiao-jun2，CHEN Si2，YANG Ping-chang2，LIU Zhi-gang2，ZHANG Min1*

Department of Respiratory Medicine，the First Affiliated Hospital of Shenzhen University，Shenzhen 518037，China；2 Allergies and Immunology Institute，Shenzhen University，Shenzhen 518060，China

Online:2015-04-30 Published:2015-05-04

摘要/Abstract

摘要：

目的探讨Th17细胞及其细胞因子白细胞介素-17（IL-17）在粉尘螨致敏哮喘小鼠中的变化及其意义。　　方法　20只BALB/c小鼠随机均分为哮喘组和健康对照组。哮喘组小鼠于第0、7和14天每鼠腹腔注射200 μl致敏液[含粉尘螨粗浸液提取物50 μg，Al（OH）3 2 mg]致敏。末次免疫后1周采用粉尘螨粗浸液提取物连续进行滴鼻激发，每天1次，每次50 μg，激发7次，健康对照组给予等体积的PBS[含Al（OH）3 2 mg]处理。末次激发后24 h内处死小鼠，取血清和肺泡灌洗液，无菌取脾脏。ELISA检测血清中IgG1、IgE和肺泡灌洗液中IL-17的含量；流式细胞仪检测脾脏中Th17细胞百分率。　　结果　哮喘组小鼠血清中IgG1和IgE水平分别为（0.10±0.01）pg/ml和（1.15±0.10）pg/ml，高于健康对照组的（0.06±0.01）pg/ml和（0.04±0.01）pg/ml（P<0.05）；哮喘组小鼠肺泡灌洗液IL-17水平（85.13±2.36）pg/ml高于健康对照组（48.27±4.14）pg/ml（P<0.01）；哮喘组小鼠脾脏Th17细胞百分率（5.19±0.68）%高于健康对照组（0.95±0.19）%（P<0.01），且脾脏Th17细胞百分率与肺泡灌洗液中IL-17水平呈正相关（r=0.851，P<0.01）。　结论　粉尘螨致敏哮喘小鼠较健康小鼠肺泡灌洗液IL-17水平和脾脏Th17细胞数量均升高。

Abstract:

Objective To detect the percentage of Th17 cells in spleen and IL-17 level in bronchoalveolar lavage fluid in Dermatophagoides farinae allergic asthma mice. Methods Twenty BALB/c mice were randomly divided into control group（n=10） and asthma group（n=10）. Mice in control group were treated with PBS plus 2 mg Al（OH）3 and those in asthma group were sensitized with 200 μl solution［50 μg Dermatophagoides farinae crude extracts plus 2 mg Al（OH）3］ on day 0， 7 and 14. One week after the last sensitization， all mice were intranasally challenged with 50 μg Dermatophagoides farinae crude extracts daily for 7 days. Twenty-four hours after the last challenge， mice were sacrificed. The sera， bronchoalveolar lavage fluid（BALF） and spleens were collected. The serum levels of IgE and IgG1， and IL-17 level in BALF were determined by ELISA. The percentage of Th17 cells in spleen was tested by flow cytometry. Results The serum levels of IgG1 and IgE in asthma group were（0.10±0.01） pg/ml and （1.15±0.10） pg/ml， respectively， which were higher than that of the control［（0.06±0.01） pg/ml and （0.04±0.01） pg/ml］（P<0.05）. IL-17 level in asthma group（85.13±2.36） pg/ml was higher than that of the control[（48.27±4.14） pg/ml]（P<0.01）. The percentage of Th17 cells in asthma group[（5.19±0.68）%] was also higher than that of the control[（0.95±0.19）%]（P<0.01）. Meanwhile， the percentage of Th17 cells in spleen was positively correlated with IL-17 level in BALF（r=0.851， P<0.01）. Conclusion Compared with healthy mice， both the percentage of Th17 cells in spleen and IL-17 level in BALF have increased significantly in Dermatophagoides farinae allergic asthma mice.

Key words: Asthma, Dermatophagoides farinae, Th17, IL-17, Mouse

吕勤1，杨小猛2，肖小军2，陈思2，杨平常2，刘志刚2，张敏1*. Th17细胞及其细胞因子白细胞介素-17在粉尘螨致敏哮喘小鼠中的变化[J]. 中国寄生虫学与寄生虫病杂志.

LV Qin1，YANG Xiao-meng2，XIAO Xiao-jun2，CHEN Si2，YANG Ping-chang2，LIU Zhi-gang2，ZHANG Min1*. Percentage of Th17 Cells in Spleen and IL-17 Level in Bronchoalveolar Lavage Fluid in Dermatophagoides farinae Allergic Asthma Mice[J]. Chinese Journal of Parasitology and Parasitic Diseases.