中国寄生虫学与寄生虫病杂志 ›› 2014, Vol. 32 ›› Issue (2): 19-161-162.

• 研究简报 • 上一篇    下一篇

武汉市美洲大蠊体内蠊缨滴虫感染情况调查

杨杰熙,唐悠远,方正明,童贞珍,李雍龙,王婷*   

  1. 华中科技大学同济医学院寄生虫学教研室, 武汉 430030
  • 出版日期:2014-04-30 发布日期:2014-07-03

Investigation on Lophomonas blattarum Infection in Periplaneta americana in Wuhan City

YANG Jie-xi,TANG You-yuan,FANG Zheng-ming,TONG Zhen-zhen,LI Yong-long,WANG Ting*   

  1. Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • Online:2014-04-30 Published:2014-07-03

摘要: 为调查武汉市美洲大蠊感染蠊缨滴虫(Lophomomas blattarum)的情况,解剖镜下分离110只美洲大蠊成虫肠道,用0.6%氯化钠溶液洗涤剪开的肠道,收集洗涤液,涂片并作吉氏染色,显微镜(×1000)下鉴定。结果显示,共44份肠道洗涤液涂片中发现蠊缨滴虫疑似物。虫体呈卵圆形或梨形,体长20~40 μm,一端有成簇的多根鞭毛。经固定、染色后, 可见虫体胞浆呈蓝色;细胞核1个,呈紫褐色,靠近虫体前端;轴柱在虫体前端呈萼状(轴罩),胞核位于其中,轴柱向后延伸至虫体末端,经鉴定确认为副基体门动鞭毛纲超鞭毛目中的蠊缨滴虫。提示武汉市美洲大蠊的蠊缨滴虫感染率为40.0%(44/110)。

关键词: 蠊缨滴虫, 美洲大蠊, 感染率

Abstract: An investigation of Lophomonas blattarum infection in Periplaneta americana in Wuhan City were conducted. A total of 110 P. americana were dissected and the intestines were separated. The intestines were washed with 0.6% saline and the washing solutions were smeared on slides. The slides were stained with Giemsa stain and observed under a microscope(×1000). Out of 110 intestine washing solution samples, 44 were suspected of L. blattarum infection. The parasite was oval or pyriform in shape and 20-40 μm in size. A tuft of flagella extended down the central axis of the parasite and a trumpet-shaped calyx enveloped the flagellar area and the nucleus. An axostyle was slender and pointed posterior ends. Based on the above morphological characteristics, the parasite was identified as L. blattarum. The results showed that the infection rate of L. blattarum in P. amerivana in Wuhan City was 40.0% (44/110).

Key words: Lophomonas blattarum, Periplaneta americana, Infection rate