中国寄生虫学与寄生虫病杂志 ›› 2013, Vol. 31 ›› Issue (3): 20-244-封三.

• 研究简报 • 上一篇    下一篇

转座酶对piggyBac转座子在弓形虫内转座活性的影响

宋鑫帅1,魏峰1 *,张莹光1,曹莉莉2,刘全2   

  1. 1 吉林农业大学生命科学学院,长春 130118;2 军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,长春 130122
  • 出版日期:2013-06-30 发布日期:2013-07-17

Effect of Transposase on the Transposition Activity of piggyBac Transposon Transfected into Toxoplasma gondii

SONG Xin-shuai1,WEI Feng1 *,ZHANG Ying-guang1,CAO Li-li2,LIU Quan2   

  1. 1 College of Life Science,Jilin Agricultural University,Changchun 130118,China;2 Military Veterinary Institute,Academy of Military Sciences of PLA;Key laboratory of Jilin Province for Zoonosis Prevention and Control,Changchun 130122,China
  • Online:2013-06-30 Published:2013-07-17

摘要: 为研究转座酶(PBase)对转座子piggyBac在弓形虫细胞内转座效率的影响,将转座酶质粒Toxo-PBase和带红色荧光蛋白(RFP)的转座子质粒PB-Toxo-RFP共同电转染刚地弓形虫RH株速殖子,流式细胞仪检测转染后的弓形虫红色荧光蛋白的转座效率。结果显示,PB-Toxo-RFP和Toxo-PBase共转染组转座效率为73%,PB-Toxo-RFP转染组转座效率为43%,前组显著高于后组(P<0.01),表明转座酶能够提高piggyBac转座子在弓形虫细胞内的转座效率。

关键词: piggyBac转座子, 转座酶, 转座效率, 刚地弓形虫

Abstract: To determine the transfection efficiency about PBase to piggyBac transposon in transfecting to Toxoplasma gondii, T. gondii RH strain tachyzoites were transfected with plasmid PB-Toxo-RFP which was expressed piggyBac transposon with a red fluorescent protein and Toxo-PBase plasmid which is a transposable enzyme. T. gondii tachyzoites were transfected with PB-Toxo-RFP plasmid alone as control group. The expression of red fluorescent protein was detected by flow cytometry at 24 h after transfection. The transposition efficiency in PB-Toxo-RFP+Toxo-PBase group and PB-Toxo-RFP group was 73% and 43%, respecitvely(P<0.01). It suggests that the PBase transposase can improve the transfection efficiency of piggyBac transposon in T. gondii tachyzoites.

Key words: piggyBac transposon, Transposase, Transposition efficiency, Toxoplasma gondii