中国寄生虫学与寄生虫病杂志 ›› 2011, Vol. 29 ›› Issue (2): 16-98-100.

• 研究简报 • 上一篇    下一篇

棘阿米巴土壤分离株CB/S1内共生细菌的16S rDNA序列分析

玄英花,崔春权,郑善子*   

  1. 延边大学基础医学院免疫学与病原生物学教研部,延吉 133000
  • 出版日期:2011-04-30 发布日期:2012-09-27

Sequence Analysis of 16S rDNA Gene of Endosymbiont of Acanthamoeba sp. CB/S1 Isolated from Soil

XUAN Ying-hua,CUI Chun-quan,ZHENG Shan-zi*   

  1. Department of Pathogenic Biology,College of Basic Medicine,Yanbian University,Yanji 133000,China
  • Online:2011-04-30 Published:2012-09-27

摘要: 用地衣红-卡红染色进行共生菌的形态观察,鉴定棘阿米巴CB/S1内共生细菌。克隆内共生细菌的16S rDNA基因,进行基因序列分析。结果表明,经地衣红-卡红染色棘阿米巴CB/S1内共生细菌呈黑色和棒状,在胞质内不规则分布。棘阿米巴CB/S1内共生细菌的16S rDNA基因长1 534 bp,与类亚洲嗜阿米巴杆菌(Candidatus Amoebophilus asiaticus 5a2)和韩国棘阿米巴分离株 KA/E21内共生细菌的16S rDNA基因的同源性均为98%。进化树分析表明,棘阿米巴CB/S1内共生细菌与韩国棘阿米巴KA/E21内共生细菌、类亚洲嗜阿米巴杆菌、黑脚硬蜱内共生细菌和伯恩蚜小蜂内共生细菌等细菌构成单系。

关键词: 棘阿米巴, 16S rDNA, 内共生细菌, 类亚洲嗜阿米巴杆菌

Abstract: The endosymbiont of Acanthamoeba sp. CB/S1 was identified by orcein-carmine staining and 16S rDNA sequence analysis. The endosymbiont bacteria were rod-shaped and darkly stained, and irregularly localized within the cytoplasm. The length of the 16S rDNA was 1 534 bp and its DNA sequence was closely related to those of Candidatus Amoebophilus asiaticus and Acanthamoeba sp. KA/E21 with 98% homology. Phylogenetic analysis showed that the endosymbiont of CB/S1, the endosymbiont of KA/E21, Candidatus Amoebophilus asiaticus, the endosymbiont of Ixodes scapularis,and the endosymbiont of Encarsia pergandiella constitute a monophyletic lineage in phylogenetic tree.

Key words: Acanthamoeba, 16S rDNA, Endosymbiont, Candidatus Amoebophilus asiaticus