关键词: 刚地弓形虫, 多表位抗原基因, 转基因番茄, 表达

Abstract: Objective To obtain the transgenic tomato plant expressed multiepitope antigenic gene of Toxoplasma gondii（Tg-MAG）. Methods Tg-MAG under the control of E35S promoter， E35S-E81.1 chimeric promoter and E8 2.2 promoter in the plant expression vectors was transferred into the tomato cotyledons and hypocotyls via Agrobacterium-mediated T-DNA transformation. The candidate buds were obtained and elongated by kanamycin resistant screening. After further rooting selection， the selected tomato lines were trained and transplanted to soil and grown in a greenhouse till blossoming and bearing fruit.The transgenic tomato plants were identified using PCR， RT-PCR and Western blotting. Results Tg-MAG was introduced into the transgenic plant genomic DNA and transcribed correctly at expected size 360 bp identified by PCR and RT-PCR. Western blotting results indicated that the recombinant Tg-MAG from 8 transgenic lines， expressed in tomato fruits at expected size 11 900， 2 of them showed strong reaction band with HRP labeled McAb K7H3 against the major surface antigen 1（ SAG1） of T. gondii tachyzoites. Conclusion Tg-MAG transgenic tomato lines were obtained.Tg-MAG recombinant protein with good immune activity was expressed successfully in transgenic tomato fruits.

Key words: Toxoplasma gondii, Multiepitope antigenic gene（MAG）, Transgenic tomato, Expression