中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (1): 18-74.

• 研究简报 • 上一篇    下一篇

旋毛虫对昆明小鼠最小感染剂量的实验研究

崔晶*, 王洁, 王中全, 牛洪涛   

  1. 郑州大学医学院寄生虫学教研室, 郑州 450052
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-02-28 发布日期:2008-02-28
  • 通讯作者: 崔晶

Minimum Dose of Trichinella spiralis Muscle Larvae to Infect Mice

CUI Jing*, WANG Jie, WANG Zhong-qan, NIU Hong-ao   

  1. Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-02-28 Published:2008-02-28
  • Contact: CUI Jing

摘要: 为观察旋毛虫感染昆明小鼠的最小剂量, 将70只雄性昆明小鼠随机均分7组, 每只鼠分别经口感染旋毛虫肌幼虫30、 25、 20、 15、 10、 5及3条, 感染后6周剖杀。取完整膈肌, 压片、 镜检、 计数虫荷。小鼠全身肌肉经人工胃液消化后计数肌肉虫荷。结果, 压片镜检法和人工消化法的幼虫检出率, 感染30、 25、 20、 15及10条旋毛虫肌幼虫的小鼠均为100% (10/10); 感染5条旋毛虫肌幼虫的小鼠, 两种方法幼虫检出率分别为70% (7/10) 和100% (10/10); 感染3条旋毛虫肌幼虫的小鼠, 两种方法均未检出幼虫。感染旋毛虫的小鼠膈肌和肌肉虫荷与感染剂量均呈正相关(r=0.759, P<0.05; r=0.638, P<0.05)。旋毛虫经口成功感染昆明小鼠的最小剂量为5条幼虫。

关键词: 旋毛虫, 染剂量, 小鼠

Abstract: Seventy male mice (Kunming strain) were randomly divided into 7 groups(10 mice per group), each mouse was orally inoculated with 30, 25, 20, 15, 10, 5 or 3 muscle stage larvae of Trichinella spiralis, respectively. All infected mice were sacrificed 6 weeks post-noculation, number of larvae per gram (LPG) of diaphragm were counted by compression method (trichinelloscopy), the carcass was digested by artificial digestion method and LPG was counted. The larval detection rate by trichinelloscopy and digestion method was 100%(10/10) in all mice infected with 30, 25, 20, 15 or 10 larvae, but 70% (7/10) and 100% in mice infected with 5 larvae, respectively. No larva was found by either method in mice infected with 3 larvae. There is a positive correlation between the larval burden (of diaphragm and muscle) and the infecting dose ( r=0.759, P<0.05; r=0.638, P<0.05), and the minimum infection dose to a mouse is 5 larvae.

Key words: Trichinella spiralis, Infection dose, Mouse