中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (1): 16-69.

• 研究简报 • 上一篇    下一篇

补骨脂与双氢青蒿素合剂治疗小鼠隐孢子虫病的实验研究

陈玉凤1, 秦元华1,  郑莉莉1,  戴晓冬1, 任一鑫1, 聂大平2,  崔昱1 *   

  1. 1 大连医科大学寄生虫学教研室, 大连, 116044; 2 大连医科大学附属二院检验科, 大连, 116023
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-02-28 发布日期:2008-02-28
  • 通讯作者: 崔昱

Experimental Treatment Using Combined Fructus psoraleae and Dihydroartemisinin in Mouse Cryptosporidiosis

CHEN Yu-feng1, QIN Yuan-hua1, ZHENG Li-li1, DAI Xiao-dong1,
REN Yi-xin1, NIE Da-ping2, CUI Yu1 *
  

  1. 1 Department of Parasitology, Dalian Medical University, Dalian 116027, China; 2 Clinical Laboratory, The 2nd Affiliated Hospital of Dalian Medical University, Dalian 116023, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-02-28 Published:2008-02-28
  • Contact: CUI Yu

摘要: 用补骨脂和双氢青蒿素合剂治疗经地塞米松诱导的隐孢子虫病小鼠, 剂量为补骨脂3.4 g/kg, 双氢青蒿素60 mg/(kg·d), 灌胃, 连用7 d。 结果粪检隐孢子虫卵囊数、 全血CD4+、 CD3+ T细胞比例、 血清γ干扰素 (IFN-γ) 浓度, 以及小肠组织匀浆一氧化氮 (NO) 浓度均高于感染对照组 (P值均<0.01)。小肠组织病变程度均轻于感染对照组。表明补骨脂和双氢青蒿素合剂可通过调节血清IFN-γ、 全血CD4+、 CD3+ T 细胞比例, 以及提高肠组织NO浓度, 参与宿主免疫应答和炎症反应过程, 抑杀虫体、 治疗小鼠隐孢子虫病。

关键词: 补骨脂, 双氢青蒿素, 隐孢子虫病, γ干扰素, T细胞, 一氧化氮

Abstract: A combination of Fructus psoraleae (3.4 g/kg) and dihydroartemisinin (60mg/(kg·d)) was used for 7 d in mice infected with Cryptosporidium parvum infection induced by oral administration of dexamethasone. The results indicated that number of Cryptosporidium parvum oocysts in feces, proportion of CD4+, CD3+ T lymphocytes in blood, serum IFN-γ, and nitric oxide (NO) content in intestinal tissue were all higher than those of infected control group (P<0.01). The pathogenic change in intestine of treated mice was lighter than that of infected control. It is indicates that the combination of Fructus psoraleae and dihydroartemisinin is effective to mice cryptosporidiosis probably through up-regulating serum IFN-γ, proportion of CD4+ and CD3+ T cells and NO concentration in intestine.

Key words: Fructus psoraleae, Dihydroartemisinin, Cryptosporidium parvum, IFN-γ, T lymphocyte, Nitric oxide