中国寄生虫学与寄生虫病杂志 ›› 2006, Vol. 24 ›› Issue (5): 6-248.

• 论著 • 上一篇    下一篇

腔阔盘吸虫18S rRNA及亲缘关系分析

郑亚东1;骆学农1;施程洪1;宗瑞谦2;景志忠1;才学鹏1   

  1. 1 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室, 兰州 730046; 2 甘肃农业大学 动物医学院, 兰州 730070
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-10-30 发布日期:2006-10-30
  • 通讯作者: 才学鹏

Molecular Relationship of Eurytrema coelmaticum Inferredfrom 18S rRNA Sequence

ZHENG Ya-dong1;LUO Xue-nong1;SHI Cheng-hong1;ZONG Rui-qian2;
JING Zhi-zhong1;CAI Xue-peng1
  

  1. 1 Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology,CAAS,Lanzhou 730046,China;2 College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730046,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-10-30 Published:2006-10-30
  • Contact: CAI Xue-peng

摘要: 目的 利用分子生物学技术,研究腔阔盘吸虫的分类。 方法 提取腔阔盘吸虫基因组DNA,利用保守引物,PCR扩增18S rRNA片段并测序。应用DNAStar和MEGA3软件,分析腔阔盘吸虫与其他双腔吸虫18S rRNA的同源性,在此基础上绘制出系统进化树,确定它们的进化关系。 结果 腔阔盘吸虫和其他双腔吸虫18S rRNA的同源性很高,其中腔阔盘吸虫和支双腔吸虫(D. dendriticum)的差异最大,为2.42%,而与愁体吸虫(L. collurionis)的差异较小,为1.75%; D. dendriticum和分叶短腺吸虫(B. lobatum)的进化关系相对较近,差异为1.09%。 结论 双腔科吸虫的18S rRNA序列非常保守,腔阔盘吸虫和L. collurionis的亲缘关系最近,而与B. lobatumD. dendriticum在进化上关系相对较远。

关键词: 腔阔盘吸虫, 双腔科, 18S rRNA, 分子分类

Abstract: Objective To elucidate the taxonomic position of Eurytrema coelmaticum by using molecular technology. Methods 18S rRNA fragment was amplified from E. coelmaticum genomic DNA by specific conservative primers and sequenced. Homology and phylogenic tree of 18S rRNA sequences between E. coelmaticum and other Dicr-ocoeliidae trematodes were analyzed and constructed by DNAStar and MEGA3 respectively, and their evolutionary relationship was determined. Results E. coelmaticum 18S rRNA sequence was with high homology to those from Dicrocoelium dendriticum, Lyperosomum collurionis and Brachylecithum lobatum. Among them, the diversity of E. coelmaticum from D. dendriticum was 2.42%, and that from L. collurionis was 1.75%; D. dendriticum and B. lobatum were closer in evolution only with 1.09% diversity. Conclusion For Dicrocoeliidae trematodes, classification based on 18S rRNA target is valid and the sequences are highly conservative. E. coelmaticum is evolutionarily closer to L. collurionis than to D. dendriticum and B. lobatum.

Key words: Eurytrema coelmaticum, Eurytrema coelmaticumDicrocoeliidae, 18S rRNA, Molecular classification