中国寄生虫学与寄生虫病杂志 ›› 2004, Vol. 22 ›› Issue (2): 5-85.

• 论著 • 上一篇    下一篇

抗环子孢子蛋白保守II+区单克隆抗体的制备及鉴定

张瑞娟,朱淮民,李翔宇   

  1. 第二军医大学病原生物学教研室,上海200433
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-04-30 发布日期:2004-04-30
  • 通讯作者: 朱淮民

Preparation and Identification of Monoclonal Antibodies against the Region II+ Motif in Circumsporozoite Protein of Plasmodium falciparum

ZHANG Rui-juan,ZHU Huai-min,LI Xiang-yu   

  1. Department of Etiological Biology,Second Military Medical University,Shanghai 200433,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-04-30 Published:2004-04-30
  • Contact: ZHU Huai-min

摘要: 目的 获得针对恶性疟原虫环子孢子蛋白 (CSP)保守II+ 区 (RegionII+ )的单克隆抗体。 方法 采用恶性疟原虫保守II+ 区十二肽 (EWSPCSVTCGNG)免疫BALB/c小鼠 ,经融合 ,ELISA 3次筛选 ,获得 3株分泌针对保守II+ 区单克隆抗体的杂交瘤细胞株。 结果 ELISA检测结果显示单抗能与重组表达的恶性疟原虫CSP片段及天然CSP特异性反应。间接荧光抗体检测显示 ,单抗不仅能识别恶性疟原虫子孢子 ,也能识别约氏疟原虫子孢子。 结论 成功地获得了针对恶性疟原虫CSP保守II+ 区的单克隆抗体。

关键词: 疟原虫属, 单克隆抗体, 环子孢子蛋白, 保守II+

Abstract: Objective To develop and identify the monoclonal antibodies (McAbs) against Region II+ motif in circumsporozoite protein of Plasmodium falciparum. Methods BALB/c mice were immunized with 12 peptides within Region Ⅱ+ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained. Results ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II+.IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii. Conclusion McAbs obtained can probe the Region II+ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species.

Key words: Plasmodium, monoclonal antibody, circumsporozoite protein, Region II+