中国寄生虫学与寄生虫病杂志 ›› 2003, Vol. 21 ›› Issue (5): 11-295.

• 论著 • 上一篇    下一篇

斯氏按蚊血淋巴蛋白浓度的检测及其意义

杨松,黄复生,况明书,段建华,张敬如   

  1. 第三军医大学基础医学部病原生物学教研室,重庆 400038
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-10-30 发布日期:2003-10-30

Assay of Haemolymph Protein Concentration in Anopheles stephensi

YANG Song,HUANG Fu-sheng,KUANG Ming-shu,DUAN Jian-hua,ZHANG Jing-ru   

  1. Department of Pathogenic Biology,Faculty of Basic Medicine,Third Military Medical University,Chongqing 400038
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-10-30 Published:2003-10-30

摘要:   目的 了解斯氏按蚊成蚊在不同食源条件下血淋巴蛋白浓度的变化。 方法 挤压法分别收集4组(吸蔗糖水组、吸正常血组、吸感染血组和吸硝喹组)斯氏按蚊成蚊血淋巴,采用Bradford法测定血淋巴蛋白浓度,并进行Excel自动分析。 结果 吸血感染约氏疟原虫8d后,吸感染血组血淋巴蛋白浓度高于吸蔗糖水组和吸正常血组,平均分别为4.436、3.080和3.092μg/μl,通常硝喹组浓度(2.264μg/μl)低于吸感染血组。 结论 吸硝喹组蚊体内血淋巴蛋白浓度下降,提示蚊血淋巴蛋白可能参与疟原虫卵囊的黑化包裹反应。

关键词: 血淋巴蛋白, 斯氏按蚊, 约氏疟原虫

Abstract:  Objective To ascertain the changes of haemolymph protein concentration in adult Anopheles stephensi mosquitoes under different feeding conditions. Methods Haemolymph samples from four groups of adult An.stephensi, fed with sucrose solution, normal blood, plasmodium-infected blood and nitroquine, respectively, were collected by expulsion method. The concentration of haemolymph protein was examined by Bradford method. The results were analyzed automatically by Excel program. Results The level of protein concentration in the infected blood-fed group was higher than the sucrose solution group and normal blood group at day 8 after Plasmodium yoelii infection,the average concentration was 4.436,3.080 and 3.092 μg/μl,respectively. The haemolymph protein concentration (2.264 μg/μl) in the nitroquine-administered mosquitoes was lower than the infected blood-fed mosquitoes. Conclusion The haemolymph protein concentration of the adult An. stephensi decreases after the nitroquine administration, indicating that the haemolymph proteins may be involved in the melantotic encapsulation reaction of plasmodial oocysts.

Key words: haemolymph protein, Anopheles stephensi, Plasmodium yoelii