中国寄生虫学与寄生虫病杂志 ›› 2003, Vol. 21 ›› Issue (3): 13-172.

• 实验报道 • 上一篇    下一篇

新疆株细粒棘球绦虫不同发育阶段95抗原基因克隆及序列分析

林仁勇,丁剑冰,温浩,张文宝,李君,卢晓梅
  

  1. 新疆医科大学第一附属医院新疆包虫病基础医学重点实验室;新疆医科大学免疫学教研室,昆士兰医学研究所,乌鲁木齐 830054,澳大利亚布里斯班 4029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-06-30 发布日期:2003-06-30

Cloning and Sequence Analysis of Eg95 cDNA from Different Stages of Echinococcus granulosus in Xinjiang

LIN Ren-yong,DING Jian-bing,WEN Hao,ZHANG Wen-bao,LI Jun,LU Xiao-mei
  

  1. 1 Xinjiang Hydatid Fundamental Medical Key Lab,The 1st Teaching Hospital of Xinjiang Medical University,Urumqi 830054 2 Department of Immunology,Xinjiang Medical University,Urumqi 830054 3 Queensland Institute of Medical Research,Brisbane,QLD,4029
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-06-30 Published:2003-06-30

摘要:   目的研究细粒棘球绦虫95抗原(Eg95)基因在新疆株细粒棘球绦虫不同发育阶段的表达及其基因序列差异。方法根据Eg95基因序列设计引物,用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段(原头蚴、六钩蚴和成虫)所构建的cDNA文库中克隆获得Eg95目的基因,将其克隆至pUCm-T载体、测序并进行序列分析。结果从3个不同发育阶段的新疆株细粒棘球绦虫cDNA文库中均克隆出Eg95基因,其基因片段长度为402bp,同源性比对分析(BLAST)结果表明,所克隆的新疆株Eg95基因与GenBank中的Eg95基因序列一致。结论Eg95基因在新疆株细粒棘球绦虫不同发育阶段均有表达,其基因序列无差异。

关键词: 细粒棘球绦虫, 细粒棘球绦虫95抗原基因, 序列分析

Abstract:  Objective To study expression and sequence differences of Echinoaxcus granulosus 95(Eg95) antigen cD-NA from different stages of protoscolex, oncosphere and adult worm of E. granulosus from Xinjiang Uighur Aut. Reg. Methods In accordance with the sequence of Eg95 antigen cDNA, the primers of Eg95 were designed. Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware. Results PCR results showed that Eg95 antigen cDNA was amplified from three stages of E. granulosus cDNA libraries. Sequencing analysis indicated that the Eg95 cDNA length was 402 bp, same as the reported data in GenBank. Conclusion The Eg95 antigen cDNA was expressed in the different life-cycle stages of E. granulosus in Xinjiang and there was no nucleic acid sequence difference of Eg95 antigen among the protoscolex, oncosphere and adult worm of E. granulosus.

Key words: Echinococcus gronu/osus, Eg95 antigen cDNA, sequence analysis