中国寄生虫学与寄生虫病杂志 ›› 2002, Vol. 20 ›› Issue (1): 9-34.

• 论著 • 上一篇    下一篇

序列标签法寻找日本血吸虫新基因

李焱,余新炳,吴忠道,李晖婷,包俊英,邵筱
  

  1. 中山医科大学寄生虫学教研室 广州510080
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-28 发布日期:2002-02-28

Obtaining and Construction of a Novel Gene from Schistosoma japonicum by Expressed Sequence Tags

LI Yan;YU Xin-bing;WU Zhong-dao;LI Hui-ting;BAO Jun-ying;SHAO Xiao
  

  1. Department of Parasitology;Sun Yat-Sen University of Medical Sciences;Guangzhou 510080
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-28 Published:2002-02-28

摘要:   目的 寻找日本血吸虫新的疫苗候选分子 ,通过构建基因的反义核酸表达载体进行基因性质功能研究。 方法 运用表达序列标签法寻找并获得日本血吸虫新基因 ,对未知基因进行功能分析 ,并构建反义真核表达载体。 结果 获得表达序列标签 JAYL0 2 30的全长 c DNA序列 ,其推导氨基酸序列与人类和猪等生物的抗凋亡因子 1具有同源性 ,将之反向克隆入真核表达载体 p EGFP- N3中。 结论 表达序列标签法与生物信息技术结合有利于寻找新基因 ,反义核酸载体的构建为进一步研究基因功能提供帮助

关键词: 日本血吸虫, 表达序列标签, 抗凋亡因子1Dad1), 反义核酸, 克隆

Abstract:  Objective To look for new candidate vaccine molecule and characterize new genes from Schistosoma japonicum. Methods New genes of S.japonicum were obtained through expressed sequence tags and analyzed on line. One interest cDNA was cloned into anti-sense eukaryotic expression vector. Results An interest cDNA JAYL0230 was obtained. Analysis showed that homology existed between its deduced amino acid sequence and defender against apoptotic death 1 of human and pig, etc. Its anti-sense cDNA was cloned into eukaryotic expression vector pEGFP-N3. Conclusion The combination of expressed sequence tags and bioinformatics is an effective way to find novel genes of creature. Construction of anti-sense eukaryotic vector is helpful in studying gene′s function.

Key words: Schistosoma japonicum, expressed sequence tags, defender against apoptotic death1Dad1, anti-sense RNA, clone