关键词: 蕊甲醚, 日本血吸虫, 特代谢, 同工酶

Abstract: Abstract [Objective] To study thee ffecto far temetherons everale nzymesi nvolved inc arbohydratem etabolism of Schistooma japonicum. [Methods] Mice infected with Schistosoma japonicum cercariae for 4-5 weeks were administered intragastrically with artemether 300 mg/kg and killed 24一72 h after medication. The supernatant fluids of female and male worm homogenates were prepared for determining 9 essential enzymes of carbohydrate metabolism by using horizontal starchgel electrophoresis, [Results] The activities of 8 out of 9 enzymes (i. e. hexokinase, aldolase, glucosephophate isomerase, malate dehydrogenase, malic enzyme, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and mannose- 6-phosphate isomerase) in female worms from artemether-treated mice were obviously inhibited 24~72 h after treatment. In male worms, only aldolase，mannose-6-phosphate isomerase and glucose 6-phosphate dehydrogenase were slightly inhibited. [Conclusion] Artemether displayed apparent effects on the carbohydrate metabolism of female schistosome

Key words: Words: Artemether, Schistosoma /aponicum, carbohydrate metabolism, isoenzyme