中国寄生虫学与寄生虫病杂志 ›› 2000, Vol. 18 ›› Issue (2): 6-86.

• 论著 • 上一篇    下一篇

齿龈内阿米巴的连续培养与致病性研究

陈金富1;刘光英1;温旺荣2;陈超3
  

  1. 福建医科大学寄生虫学教研室!福州350004(陈金富;刘光英);福建医科大学临床微生物学教研室!福州350004(温旺荣);福建医科大学附属口腔医院!福州350004(陈超)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-04-30 发布日期:2000-04-30

STUDIES ON THE CONTINUOUS CULTURE AND PATHOGENICITY OF ENTAMOEBA GINGIVALIS

CHEN Jin-fu1;LIU Guang-ying1;WEN Wang-rong2;CHEN Chao3   

  1. 1 Department of Parasitology;Fujian Medical University;Fuzhou 350004; 2 Department of Clinical Microbiology;Fujian Medical University;Fuzhou 350004; 3 Affiliated stomatology hospital,Fuji Medical University,Fuzhou 350004
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-04-30 Published:2000-04-30

摘要:   [目的 ]建立齿龈内阿米巴 (Entamoeba gingivalis,E.g.)的连续培养方法和研究虫体的致病性。 [方法 ]比较不同培养条件下虫体生长繁殖情况 ,调查人群感染 E.g.情况及观察感染 E.g.与唾液中酸性磷酸酶含量的关系 ,大鼠实验观察 E.g.感染与牙周脓肿的关系 ,E.g.体外实验观察甲硝唑的杀虫效果。 [结果 ]虫体最适培养条件为 :改良的 L ES培养基中加含 2 0 %小牛血清的 L ocke液、青霉素、链霉素和米粉 ,p H6 .7于 35℃培养 ,第4天虫体繁殖达高峰 ,可存活 12 0~ 16 8h;E.g.检测阳性的牙周病患者其酸性磷酸酶和脂质过氧化的终产物(MDA)含量高于阴性者 ,牙周脓肿患者 E.g.感染率高于健康人群。E.g.可致大鼠牙周脓肿 ,甲硝唑与绣球花叶(SBF)杀 E.g.有良效。 [结论 ]E.g.在改良的 L ES培养基中生长良好 ,当宿主免疫力低下时 E.g.可致牙周脓肿

关键词: 齿龈内阿米巴, 培养, 动物感染, 牙周脓肿

Abstract:  Objective] To establish a method for continuous culture of Entamoeba gingivalis (E.g.) and study the pathogenicity of E.g.. [Methods] The culture conditions of E.g. were compared by observing its growth and reproduction. The pathogenicity of E.g. was studied by investigating its infection rate in humans and observing the relationship between E.g. infection and periodontal abscess in rats and the in vitro effect of some drugs. [Results] The optimal culture condition of E.g. was modified LES medium containing Locke′s solution with 20% bovine serum, penicillin, streptomycin and rice starch. Reproduction of E.g. peaked at the fourth day of incubatoin, E.g. could live continuously for 120 to 168 h. The infection rate of E.g. was higher in patients with periondontal diseases than in healthy groups. The animal test showed that E.g. could cause periondontal abscess in rats. The levels of acid phosphatase and MDA were higher in patients with E.g.-positive periondontal disease than in healthy individuals. Metronidazole and SBF are effective against E.g.. [Conclusion] E.g. could grow very well in modified LES medium, and cause periondontal abscess in patients with low immunity. \;

Key words: Entamoeba gingivalis, culture, animal infection, periodontal abscess.