中国寄生虫学与寄生虫病杂志 ›› 2000, Vol. 18 ›› Issue (2): 13-112.

• 实验报道 • 上一篇    下一篇

棘球蚴感染长爪沙鼠诱发过敏反应的实验观察

郑宏;温浩;徐志新;张朝霞;尹极峰
  

  1. 新疆医科大学第一附属医院!乌鲁木齐830054
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-04-30 发布日期:2000-04-30

EXPERIMENTAL OBSERVATION ON ANAPHYLAXIS INDUCED BY ECHINOCOCCUS INFECTION IN MERIONES UNGUICULATUS

ZHENG Hong;WEN Hao;XU Zhi-xin;ZHANG Zhao-xia;YIN Ji-feng   

  1. Department of Anesthesiology;Hydatid Research Unit;First Teaching Hospital;Xinjiang Medical University;Urumqi 830054
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-04-30 Published:2000-04-30

摘要:   [目的 ]观察棘球蚴感染长爪沙鼠诱发的过敏反应。 [方法 ]取 5 6只及 48只长爪沙鼠 ,分别腹腔接种感染细粒棘球蚴 E.g.及泡状棘球蚴 E.m.,3个月后分别用羊源新鲜囊液、羊源 E.g.粗制囊液抗原及沙鼠 E.m.抗原经腹腔注射攻击 ,观察其过敏反应。在攻击发敏前后 ,检测 Ig E抗体水平和嗜酸性粒细胞直接计数 (directeosinophilic leukocyte count,DEL C)。 [结果 ]沙鼠 E.g.和 E.m.感染率分别为 89.3%和 97.9%。各组过敏反应发生率最低为 6 2 .5 % ,最高为 10 0 %。过敏性休克发生率最低为 12 .5 % ,最高为 12 .7%。沙鼠感染 E.g.和 E.m.后 ,Ig E抗体和 DEL C水平逐渐增高 ,但攻击发敏后 Ig E抗体水平显著降低 ,DEL C显著增多。 [结论 ]E.g.和 E.m.感染沙鼠后 ,采用不同抗原攻击均可出现过敏反应或休克 ;不同抗原引起过敏反应的发生率和程度不同 ,但在感染E.g.与 E.m.沙鼠之间无显著性差异。

关键词: 棘球蚴, 长爪沙鼠, 抗原, 过敏反应

Abstract:  Objective] To observe the anaphylaxis induced by Echinococcus granulosus or E.multilocularis (E.g. and E.m.) infection in Meriones unguiculatus. [Methods] 56 and 48 gerbils were intraperitoneally inoculated with E.g. protoscoleces and E.m. tissue homogenate. After three months, the anaphylactic responses initiated by intraperitoneal challenge injection with E.g. cystic fluids or partially purified antigen or E.m. metacestode homogenate antigen were observed. IgE antibody and direct eosinophilic leukocyte count (DELC) were detected at pre-postchallenge. [Results] The infection rates for E.g and E.m.in 56 and 48 gerbils were 89.3% and 97.9%, respectively. Among these groups, the lowest rate of anaphylaxis was 62.5%, the highest was 100%. The lowest rate of anaphylactic shock was 12.5%, and the highest 16.7%. After the gerbis were infected with E.g. and E.m., both IgE antibody and DELC increased gradually. At 60 min post challenge, IgE antibody level were significantly decreased, while DELC showed a sharp increase. [Conclusion] Anaphylaxis could be induced in gerbils by Echinococcus infection followed by a challenge injection. Different antigens lead to different and degrees among the anaphylactic responses in the gerbils, however, there were no significant differences between E.g. and E.m. infections in gerbils.

Key words: Echinococcus, Meriones unguiculatus, Anaphylaxis.