中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (6): 5-341.

• 论著 • 上一篇    下一篇

恶性疟原虫单表位单抗的建立及鉴定

瞿靖琦1;杨柏林1;包意芳1;杨玥涛1;许永湘1;许学年1;范培昌2;任一萍2
  

  1. 1 中国预防医学科学院寄生虫病研究所,上海 200025 2 华东师范大学生物系 上海 200062
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-12-30 发布日期:1999-12-30

ESTABLISHMENT AND IDENTIFICATION OF THE MONOEPITOPIC MONOCLONAL ANTIBODY OF PLASMODIUM FALCIPARUM*

QU Jingqi1;YANG Bolin1;BAO Yifang1;YANG Yuetao1;XU Yongxiang1;XU Xuenian1;FAN Peichang2;REN Yiping2   

  1. 1Institute of Parasitic Diseases;Chinese Academy of Preventive Medicine;Shanghai 200025 2 East China Normal University,shanghai 200062
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-12-30 Published:1999-12-30

摘要:   目的: 研制恶性疟原虫单表位单抗。方法: 根据蛋白质结构理论, 选取能代表恶性疟原虫蛋白质抗原性的HRP-II肽段, 长度为6~9 个氨基酸, 经人工合成, 通过毛细管电泳, 确定其纯度后, 对BALB/c小鼠进行免疫。结果: 应用脾脏埋植法和杂交瘤技术, 获得4 株针对恶性疟原虫HRP-II单一表位的杂交瘤细胞。结论: 首次报告直接用合成HRP-II肽段制备针对该蛋白质的单表位单抗的方法获得成功。

关键词: 富组蛋白II, 合成肽, 单表位单抗

Abstract:  AIM: To prepare a monoepitopic monclonal antibody of Plasmoduim falciparum. METHODS: According to the theory of protein structure, a peptide with 6-9 residues representting the antigenicity of the original histidine-rich protein II (HRP-II) of P.falciparum was synthesized and used to immunize BALB/c mice after purity identification by capillary electrophoresis. RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtaind by using spleen embedment method and hybridoma technology. CONCLUSION: It is the first report to prepare a monoepitopic monoclonal antibody against original protein by selecting a suitable peptide from the primary sequence of protein.\;

Key words: Histidine-rich protein II, synthetic peptide, monoepitopic monoclonal antibody