中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (6): 18-386.

• 实验研究 • 上一篇    下一篇

紫外线减毒弓形虫ZS1株滋养体在小鼠体内的细胞免疫反应

吕芳丽;郑焕钦;郭虹;陈观今
  

  1. 中山医科大学寄生虫学研究所,广州 510089
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-12-30 发布日期:1999-12-30

CELLULAR IMMUNE RESPONSE IN MICE VACCINATED WITH UV-ATTENUATED TOXOPLASMA GONDII ZS1 STRAIN TROPHOZOITES*

LU Fangli;ZHENG Huanqin;GUO Hong;CHEN Guanjin

  

  1. Department of Parasitology;Sun Yat-Sen University of Medical Sciences;Guangzhou 510089
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-12-30 Published:1999-12-30

摘要:   目的: 探讨紫外线减毒弓形虫ZS1 株在小鼠体内的免疫保护性和细胞免疫反应。方法: 用波长为2537°A 的紫外线照射弓形虫ZS1株滋养体, 照射高度为5 cm , 照射时间60 m in。小鼠于免疫后45 d 用同株滋养体攻击感染, 攻击后4 d 剖杀, 与单免疫组、单感染组及正常对照组小鼠比较其脾T淋巴细胞增殖反应及其亚群的变化。结果: 小鼠接种紫外线减毒弓形虫ZS1 株滋养体后能正常存活, 于接种后49 d 各组织未查见滋养体、包囊或假包囊; 免疫组攻击感染后存活时间较单感染组延长; 体外特异抗原刺激后, 可诱导免疫组及免疫攻击组强的脾T淋巴细胞增殖反应; 免疫攻击组CD4+ T细胞明显下降, CD4+ /CD8+ 比率倒置; 免疫组、免疫攻击组及感染组的NK细胞活性均明显增强。结论: 紫外线减毒弓形虫ZS1株滋养体疫苗能够诱导免疫小鼠产生一定的抗攻击感染保护力, 其中CD8+ T细胞和NK细胞可能发挥着重要作用。

关键词: 弓形虫, 紫外线减毒, 脾淋巴细胞增殖, CD4~+/CD8~+T细胞, NK细胞

Abstract:  AIM: To explore the protective effect and cellular immune response of uv-attenuated ZS1 strain trophozoites of Toxoplasma gondii in mice. METHODS: The ZS1 strain trophozoites of T.gondii were irradiated by uv-light with 2537 \{°A\} wave length for 60 minutes. Mice were divided into 4 groups. Group 1 was vaccinated alone, group 2 was challenged with normal ZS1 trophozoites on d45 after vaccination, group 3 was infected alone, and group 4 was normal control. The changes in splenic T cell proliferation, level of CD4+ and CD8+ T cell, and NK cell activity were compared. RESULTS: Group1 mice survived normally, no trophozoite,pseudo-cyst or cyst was detected in the tissues on d49 after vaccination. Group 2 mice survived longer than those of group 3. The T lymphocyte proliferation in response to soluble antigen of T.gondii was significantly enhanced in group 2, and suppressed in group 3. The level of CD4+ T cell in group 2 was decreased, resulting in a reverse of CD4+/CD8+ ratio. The NK cell activities in groups 1, 2 and 3 were all significantly increased. CONCLUSION: The uv-attenuated vaccine of T.gondii ZS1 strain could induce certain protective immunity against challenge infection, in which CD8+ T cell and NK cell might play an important role.\;

Key words: Toxoplasma gondii, uv-attenuated, splenic lymphocyte proliferation, CD4+/CD8+ T cell, NK cell