关键词: 日本血吸虫, 品系, SDS-聚丙烯酰胺凝胶电泳, 酶联免疫电转移印迹

Abstract: 　AIM: To make a supplementary observation on the protein components prepared from adult S.japonicum of 4 different isolates from Zhejiang, Jiangxi, Taiwan Provinces of China and Japan in origin and to observe antigen reactivity of the above-mentioned isolates together with adult worms from Anhwi, Hubei, Sichuan and Yunnan isolates against heterologous anti- Oncomelania h.hupensis (collected from Guangxi, China and Japan) sera by EITB. METHODS: SDS-PAGE and EITB.RESULTS AND CONCLUSION: SDS-PAGE showed that by Coomassie blue staining,male S.japonicum from Jiangxi, Zhejiang, Taiwan Provinces and Japan isolales revealed 7-17 bands while female worms revealed 1-6 bands. The protein patterns of Taiwan and Zhejiang male worm were similar, but slight difference could be seen above 81 kDa. By silver staining, male worms of the 4 isolates revealed 10-23 bands while female worms revealed 1-19 bands. Male worms from Japan not only showed less bands but also differed in their pattern as compared to those of other Chinese isolates. Results of EITB revealed that each of the 4 isolates had slightly different pattern but all of the tested isolates had common antigens with their heterologous snail hosts i.e., Oncomelania h.hupensis from Guangxi, China and Japan.

Key words: Schistosoma japonicum, strain, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunoelectro-ransfer blot (EITB)