中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (6): 1-324.

• 论著 •    下一篇

日本血吸虫副肌球蛋白全基因核酸疫苗在小鼠诱生的保护性免疫力

周生华,刘述先,宋光承,徐裕信,孙文宇
  

  1. 中国预防医学科学院寄生虫病研究所 上海 200025
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-12-30 发布日期:1999-12-30

INDUCTION OF PROTECTIVE IMMUNITY IN MICE AGAINST SCHISTOSOMA JAPONICUM BY NUCLEIC ACID VACCINE ENCODING THE FULL-LENGTH PARAMYOSIN*

ZHOU Shenghua;LIU Shuxian;SONG Guangcheng;XU Yuxin;SUN Wenyu


  

  1. Institute of Parasitic Diseases;Chinese Academy of Preventive Medicine**Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-12-30 Published:1999-12-30

摘要:   目的: 研究日本血吸虫大陆株副肌球蛋白全基因核酸疫苗免疫C57 BL/6 及BALB/c 小鼠诱生的保护性免疫力。方法: 将构建的日本血吸虫大陆株副肌球蛋白全基因核酸疫苗 (pCMV-SjC97) 经后腿胫前肌免疫C57BL/6及BALB/c小鼠, 共免疫3 次, 每次间隔3 w k。末次免疫后3 w k 以血吸虫尾蚴攻击感染, 6 wk 后计数成虫负荷及肝、脾、肠组织虫卵数。设不含SjC97 编码基因的空载体质粒免疫组为对照组。结果: pCMV-SjC97 免疫C57 BL/6 小鼠主要诱生IgG2a 和IgG2b 亚类, 而免疫BALB/c小鼠除诱生IgG2a 和IgG2b 亚类外, 还诱生IgG1。pCMV-SjC97 免疫C57BL/6 小鼠诱生较明显的减虫率 (35.5% ~41.1% ) 和减卵率 (肝、脾及肠组织减卵率分别为44.5% ~59.6% 、56.7% ~82.4% 及57.9% ), 而对BALB/c小鼠未诱生保护性。结论: pCMV-SjC97 核酸疫苗能对C57BL/6 小鼠诱生较明显保护性免疫力, 而对BALB/c 小鼠未诱生免疫保护力

关键词: 日本血吸虫, 副肌球蛋白, 核酸疫苗, 保护性免疫力

Abstract:  AIM: To investigate the immune efficacy of nucleic acid vaccination in mice against full-length paramyosin of Chinese Schistosoma japonicum. METHODS: C\-\{57\}BL/6 and BALB/c mice were vaccinated intramuscularly with the nucleic acid vaccine (pCMV-SjC97) encoding the full-length gene of paramyosin of Chinese S.japonicum. Each group was immunized three times at weeks 0, 3 and 6. Mice vaccinated with pCMV blank vector served as negative control. Mice were challenged three weeks after final DNA boosting by percutaneous infection with cercariae. Six weeks after infection the mice were perfused, worm burden and eggs in the livers, spleens and intestines were counted. Sera from vaccinated mice were collected from the tail vein at weeks 0, 3, 6 and 9, respectively. RESULTS: C\-\{57\}BL/6 mice vaccinated with pCMV-SjC97 produced predominantly IgG2a and IgG2b; whereas in BALB/c mice, IgG1, IgG2a and IgG2b antibodies. Immunization with the pCMV-SjC97 in C\-\{57\}BL/6 mice could confer significant worm reduction rate (35.5%-41.4%,P<0.05) and egg reduction rate (liver:44.5%-59.6%, P<0.05; spleen:56.7%-82.4%, P<0.05; intestines:57.9%, P<0.05), but not in BALB/c mice. CONCLUSION: The nucleic acid vaccine, pCMV-SjC97, could induce protective immunity in C\-\{57\}BL/6 mice significantly. \;

Key words: Schistosoma japonicum, paramyosin, nucleic acid vaccine, protective immunity