中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (5): 16-310.

• 动物模型 • 上一篇    下一篇

家兔棘阿米巴角膜炎动物模型的建立

邓新国 1;郭雪2;庞广仁 1;田小莉1
  

  1. 河南省眼科研究所 郑州 450003;河南省卫生防疫站 郑州 450003
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-10-30 发布日期:1999-10-30

ESTABLISHMENT OF A RABBIT MODEL OF ACANTHAMOEBA KERATITIS

DENG Xinguo 1;GUO Xue 2;PANG Guangren 1;TIAN Xiaoli 1   

  1. 1 Henan Institute of Ophthalmology;Zhengzhou 450003 2 Henan Health and Epidemic Station;Zhengzhou 450003
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-10-30 Published:1999-10-30

摘要:   目的:建立棘阿米巴角膜炎的动物模型。方法:在6 只新西兰家兔角膜基质层内注射地塞米松3 d 后,再注入棘阿米巴原虫悬液。结果:6 只家兔均发生角膜炎。经家兔角膜刮片用10% 氢氧化钾封片镜检、角膜组织原虫培养和病理切片染色检查证实,成功地建立了家兔棘阿米巴角膜炎动物模型。结论:通过角膜基质内注射建立的家兔棘阿米巴角膜炎动物模型,方法简便又易于操作

关键词: 棘阿米巴角膜炎, 基质内注射, 动物模型

Abstract:  AIM: To establish an animal model of Acanthamoeba keratitis. METHODS:Six New Zealand white rabbits were each injected intrastromally with Acanthamoeba suspension 3 days after subconjunctival injection with dexamethasone. RESULTS:All of the 6 rabbits developed keratitis. Acanthamoeba protozoa were identified by the methods of corneal scraping with 10% potassium hydroxide wet mount examined under microscope,corneal protozoa culture and pathological section examination. CONCLUSION:A rabbit model of Acanthamoeba keratitis was established.

Key words: Acanthamoeba keratitis, animal model, intrastromal injection