齿龈内阿米巴的连续培养及其生长情况

中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (4): 6-211.

齿龈内阿米巴的连续培养及其生长情况

陈金富¹,刘光英¹,温旺荣²

1 　寄生虫学教研室　　福建医科大学　　　　　　　　　　　福州　350004 2 　临床微生物学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-08-30 发布日期:1999-08-30

STUDIES ON THE CONTINUOUS CULTURE AND THE GROWTH OF ENTAMOEBA GINGIVALIS

CHEN Jinfu ¹;LIU Guanyin ¹;WEN Wangrong²

1 Department of Parasitology;Fujian Medical University;Fuzhou 35004 2Department of Clinical Microbiology;Fujian Medical University;Fuzhou 350004

Received:1900-01-01 Revised:1900-01-01 Online:1999-08-30 Published:1999-08-30

摘要/Abstract

摘要： 　　目的：建立齿龈内阿米巴（Ｅｎｔａｍｏｅｂａｇｉｎｇｉｖａｌｉｓ，Ｅｇ）ＦＪ４的连续培养方法，并观察其生长情况。方法：在不同培养条件下观察Ｅｇ虫体大小及其存活时间。结果：Ｅｇ在改良的Ｌｏｃｋｅｅｇｇｓｅｒｕｍ与改良的ｙｏｌｋｅｇｇｓｅｒｕｍ培养基中生长良好，建立了福建３株Ｅｇ（ＦＪ２、ＦＪ３及ＦＪ４），虫体大小均值为１３１９ μｍ～４３９３ μｍ ×９８８ μｍ～３０７４ μｍ。Ｅｇ最适宜培养条件为ｐＨ在６４～６７、改良的Ｌｏｃｋｅｅｇｇｓｅｒｕｍ或蛋黄液含２０％小牛血清加青霉素、链霉素及米粉。虫体繁殖高峰为ｄ４，其在上述培养液体中可存活１２０ｈ～１６８ｈ。结论：Ｅｇ在Ｌｏｃｋｅｅｇｇｓｅｒｕｍ与Ｙｏｌｋｅｇｇｓｅｒｕｍ培养基中生长良好，每４ｄ转种１次，可达到长期培养的效果。

关键词: 齿龈内阿米巴, 培养条件

Abstract: 　AIM: To establish a method for continuous culture of Entamoeba gingivalis(E.g. ).METHODS:The culture conditions of E.g. were compared by observing its size and survival time at room temperature. RESULTS: The growth of E.g. under different culture conditions including culture medium, temperature and pH were compared. The accompanying bacteria associate with E.g. FJ4 were isolated and identified. The average size of E.g. was 13.19 μm ～ 49.93 μm×9.88 μm ～ 30.74 μm. The optimal culture conditions of E.g. were:modified LES or YES medium, pH 6.4 ～ 6.7, nutritional liquids such as Lockes solution or yolk liquid with 20% bovine serum, penicillin, streptomycin and rice flour at 35 ℃.Reproduction of E.g. peaked at the fourth day of incubation,and the survival time of E.g. was 120 h～168 h. CONCLUSION: E.g. could be continuously cultured in modified LES or YES medium by inoculating once every four days.

Key words: Entamoeba gingivalis, culture condition

陈金富;刘光英;温旺荣. 齿龈内阿米巴的连续培养及其生长情况[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(4): 6-211.

CHENJinfu;LIUGuanyin;WENWangrong. STUDIES ON THE CONTINUOUS CULTURE AND THE GROWTH OF ENTAMOEBA GINGIVALIS[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1999, 17(4): 6-211.

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