中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (4): 15-240.

• 实验研究 • 上一篇    下一篇

利什曼原虫抗原对草原兔尾鼠感染婴儿利什曼原虫的免疫保护作用

柴君杰 1,KwangPooChang2,左新平1,严雷1 ,侯岩岩1 ,张松1,茹孜古丽1 ,蒋卫1,张兰英1   

  1. 1  新疆地方病防治研究所、卫生部包虫病防治培训基地 乌鲁木齐 8300022  美国芝加哥医学院 芝加哥,伊利诺斯 60064
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-08-30 发布日期:1999-08-30

PROTECTIVE EFFECTS OF LEISHMANIAL ANTIGENS AGAINST LEISHMANIA INFANTUM INFECTION IN LAGURUS LAGURUS *

CHAI Junjie 1;Kwang Poo CHANG 2;ZUO Xinping1; Yan Lei 1 Hou Yanyan 1 Zhang Song 1 Ruziguli 1 JIANG Wei 1 Zhang Lanying 1   

  1. 1 National Hydatid Disease Centre of China;Institute for Endemic Diseases Control and Research of Xinjiang Uygur Autonomous Region, Urumqi,  Xinjiang 8300022  Department of Microbiology / Immunology, University of Health Sciences/ Chicago Medical School, North Chicago, Illinois 60064, USA
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-08-30 Published:1999-08-30

摘要:   目的: 测定利什曼原虫主要表面分子抗原对内脏利什曼病的免疫保护作用。方法: 用重组利什曼原虫表面糖蛋白r G P63 和脂磷酸聚糖( L P G) 为抗原, 以短棒状杆菌菌苗( C P) 为佐剂免疫草原兔尾鼠后, 用婴儿利什曼原虫强毒株攻击, 观察免疫保护效果。结果: r G P63 加 L P G 加 C P 抗原组合免疫后用2 ×107 前鞭毛体攻击, 免疫动物的肝印片上 L D 数量明显降低, 减虫率为898 % 。 L P G 加 C P 免疫组减虫率为606 % 。r G P63 包涵体加 C P 免疫组减虫率为424 % , 而纯化r G P63 加 C P 免疫组未显示保护作用。以r G P63 加 L P G 加 C P 免疫后用1 ×106 , 5 ×106 和1 ×107 前鞭毛体攻击时, 感染率亦有明显降低。结论: 利什曼原虫主要表面分子抗原 G P63和 L P G 在以 C P 为佐剂的条件下, 对草原兔尾鼠婴儿利什曼原虫有明显的免疫保护效果。

关键词: GP63, LPG, 免疫保护, 草原兔尾鼠, 内脏利什曼病, 婴儿利什曼原虫

Abstract:  AIM: : To determine the protective effect of leishmanial surface antigens against experimental visceral leishmaniasis in Lagurus lagurus caused by Leishmania infantum .METHODS: Recombinant surface protein (rGP63) and lipophosphoglycan (LPG) of Leishmania were used with Corinebacterium parum vaccine as ajuvant to immunize Lagurus lagurus against a challenge with virulent strain of Leishmania infantum . The efficacy of immunoprotection was observed.RESULTS: When challenged with up to 2×10 7 promastigotes, the number of LD on the liver printing sections in the rGP63+LPG+CP immunised animals was significantly decreased ,the parasite reduction rate being 89.79%. LPG+CP gave a parasite reduction rate of 60.6% and rGP63/β-galactosidase fusion protein +CP showed a parasite reduction rate of 42.45%.Purified rGP63 showed no protection. Immunization with rGP63+LPG+CP followed by challenge inifection with 1×10 6,5×10 6 and 1×10 7 promastigotes also showed significantly reduced infection rates.CONCLUSION: A combination of rGP63+LPG+CP antigens could provide significant immunoprotection against L.infantum challenge in L.lagurus .

Key words: GP63, LPG, immunoprotection, Lagurus lagurus Pallas, visceral leishmaniasis, Leishmania infantum