中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (1): 8-31.

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幼虫冰冻切片抗原间接荧光抗体试验诊断旋毛虫病

崔晶 1;王中全1;武峰1;晋雪香1;张鹏远2;杨瑞琴 1;刘俊3
  

  1. 1 河南医科大学寄生虫学教研室 郑州4500522 河南医科大学第二附属医院 郑州4500033 河南医科大学第一附属医院 郑州450052
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-02-28 发布日期:1999-02-28

DIAGNOSIS OF TRICHINOSIS BY INDIRECT FLUORESCENT ANTIBODY TEST WITH TRICHINELLA LARVA SECTION *

CUI Jing 1;WANG Zhongquan 1;WU Feng 1;JIN Xuexiang 1;ZHANG Pengyuan 2;YANG Ruiqin 1;LIU Jun 3   

  1. 1 Department of Parasitology;Henan Medical University;Zhengzhou 450052 2 The 2nd Affiliated Hospital;Henan Medical University;Zhengzhou 4500523The 1st Affiliated Hospital, Henan Medical University, Zheng zhou 450052
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-02-28 Published:1999-02-28

摘要:   目的:评价幼虫冰冻切片抗原间接荧光抗体试验(IFAT)诊断旋毛虫病的价值。方法:应用纯净幼虫冰冻切片间接荧光抗体试验检测感染旋毛虫小鼠和旋毛虫病患者血清中的抗旋毛虫抗体,在患者发病后及应用阿苯达唑治疗后不同时间采集血清,以其他寄生虫病患者和健康人血清为对照。结果:15只小鼠感染旋毛虫后2wk即可检出抗体,467例旋毛虫病患者的抗体阳性率为86.5%,而丝虫病、包虫病患者各45例和健康人血清56份均为阴性。48份并殖吸虫病患者的交叉反应率为16.67%。旋毛虫病患者发病后1wk的抗体阳性率为70.21%,至发病后2wk、3wk及4wk则分别升至91.0%、95.8%及100%。幼虫冰冻切片的抗原活性于-20℃可保存至5年。旋毛虫病患者治疗后1wk抗体阳性率从治疗前的87.5%上升到100%,血清抗体滴度比治疗前也有明显升高,治疗后1个月的抗体转阴率为24%,至治疗后4个月达75%。结论:幼虫冰冻切片间接荧光抗体试验诊断旋毛虫病具有较高的敏感性和特异性,并可用于疗效考核

关键词: 旋毛虫病, 血清学诊断, 间接荧光抗体试验

Abstract:  AIM: To evaluate the value of indirect fluorescent antibody test (IFAT) in diagnosing trichinosis. METHODS: IFAT using frozen section of the purified Trichinella larvae as antigen was employed to detect anti Trichinella antibodies in sera from mice infected with Trichinella spiralis and the patients with trichinosis. Sera from patients with trichinosis were collected at different time intervals after onset of the disease and after treatment with albendazole. Sera from patients with other parasitic diseases and healthy individuals were used as control. RESULTS: The anti Trichinella antibodies were detected in 15 infected mice as early as 2 weeks after infection,and in 86 5% of 467 patients with trichinosis. Sera from patients with filariasis, echinococcosis and healthy controls were all negative. 8 of 48 (16 7%) cases with paragonimiasis showed positive cross reaction. The antibody positive rate of patients with trichinosis was 70 2% one week after onset of disease and increased to 91 0%,95 8% and 100% at two, three and four weeks after onset, respectively. The frozen section of the larvae can be stored at -20℃ for 5 years without loss of antigenicity. The IFAT positive rate was increased from 87 5% before treatment to 100% one week after treatment, and the serum antibody titers were elevated as well. The antibody negative conversion rate of the patients was 24% at one month after teratment and increased to 75% at four months after treatment. CONCLUSION: IFAT using frozen section of the purified Trichinella larvae as antigen is highly sensitive and specific for the diagnosis of trichinosis and evaluation of the therapeutic effect.

Key words: Trichinosis, serodiagnosis, IFAT