免疫色谱技术用于班式丝虫病诊断及丝虫病防治后期监测的实验研究

中国寄生虫学与寄生虫病杂志 ›› 1998, Vol. 16 ›› Issue (3): 168-171.

免疫色谱技术用于班式丝虫病诊断及丝虫病防治后期监测的实验研究

郑惠君; 陶增厚; 方仁丽; 常宝玉; 张玉林

贵州省寄生虫病研究所; 河南省卫生防疫站; 澳大利亚ＪａｍｅｓＣｏｏｋ大学公共卫生和热带医学系

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-06-30 发布日期:1998-06-30

APPLICATION OF IMMUNOCHROMATOGRAPHIC TEST FOR DIAGNOSIS AND SURVEILLANCE OF BANCROFTIAN FILARIASIS

Zheng Huijun ¹; Tao Zenghou ¹; Fang Renli ¹; Chang Baoyu²; Zhang Yulin²; Paul Turner ³

1 Guizhou Provincial Institute of Parasitic Diseases; Guiyang 550004 2 Henan Provincial Health and Anti epidemic Station; Zhengzhou 3　Filariasis Control Section,Department of Public Health and Tropical Medicine, James Cook University , Town sville, Queensland, Australia

Received:1900-01-01 Revised:1900-01-01 Online:1998-06-30 Published:1998-06-30

摘要/Abstract

摘要： 目的：评价免疫色谱技术（ＩＣＴ）用于班氏丝虫病诊断及丝虫病防治后期监测的效果。方法：丝虫病ＩＣＴ系采用单克隆抗体检测血中班氏丝虫抗原。结果：在１１６例班氏微丝蚴血症者中有１１１例ＩＣＴ阳性，敏感性为９５．７％。1２例马来微丝蚴血症者、３３例蛔虫感染者、２０例血吸虫感染者及６例旋毛虫感染者血清，ＩＣＴ均为阴性，表明其特异性
强；７３例晚期丝虫病患者有１８例ＩＣＴ阳性，为活动性感染，其中１６例伴有微丝蚴血症；用伊维菌素治疗３０例班氏微丝蚴血症者，治疗前ＩＣＴ阳性２９例（９６．７％）。治疗后８ｄ—１４ｄ微丝蚴全部转阴，追踪观察６个月及１２个月，分别有５例及７例微丝蚴复现，治疗后６个月用ＩＣＴ测试，除５例ＩＣＴ和微丝蚴均阳性外，８例微丝蚴阴性ＩＣＴ阳性者于疗后１２个月有５例查见微丝蚴；ＩＣＴ及常规血检法同步用于河南省柘城县候大村丝虫病防治后期监测，血检１３２人，９例微丝蚴阳性者ＩＣＴ均为阳性，血检阴性的１２３人中ＩＣＴ阳性者１例。结论：实验研究证实，ＩＣＴ法快速、简便、敏感性高和特异性强，可用于班氏丝虫病诊断、疗效考核及丝虫病防治后期监测。

关键词: 免疫色谱技术, 快速诊断, 监测, 班氏丝虫病

Abstract:

AIM: To evaluate the usefulness of immunochromatographic test (ICT) for rapid diagnosis and
surveillance of Wuchereria bancrofti infection. METHODS: The monoclonal antibody based ICT assay was used to
detect filarial antigens in the sera from bancroftian filariasis patients. RESULTS: 111 out of 116 bancroftian
microfilarmia cases showed positive ICT reaction, giving a positive rate of 95.7%. Serum samples from 12
malayian microfilaremia cases, 33 ascariasis cases, 20 schistosomiasis japonica cases and 6 trichinellosis cases were all found negative in ICT , demonstrating a specificity of 100%. In 73 chronic filariasis cases
with elephantiasis, hydrocoele, and chyluria cases, 18 showed ICT positive, among whom 16 revealed microfilarem ia, suggesting that the ICT positive cases were with active infections. Of the 30 microfilaremia cases before single dose ivermectin treatment, 29 were ICT positive (96. 7% ). A negative conversion of microfilaremia was observed in all cases 8- 14 days post-treatment. Upon following up to 6 and 12 months, microfilaremia eappeared in 5 and 7 cases, respectively. 5 out of 13 ICT positive cases were found microfilaremic at 6 month post-treatment, while among the rest of 8 , 5 were found microfilaremic at 12 month post-treatment. Parallel use of ICT with routine blood film examination in Houda village,Zhecheng County, Henan Province for a post-control surveillance survey in 132 local individuals revealed 9 microfilaremia cases were also ICT positive. Of the 123 persons w th negative blood examination, only 1 was ICT positive. CONCLUSION: ICT is a rapid and simple method with high sensitivity and specificity, and might be used for the diagnosis, efficacy evaluation as well as a tool for post-control surveillance of bancroftian filarasis.

Key words: Immunochromatographic test, rapid diagnosis, surveillance, bancroftian filariasis

郑惠君;陶增厚;方仁丽;常宝玉;张玉林. 免疫色谱技术用于班式丝虫病诊断及丝虫病防治后期监测的实验研究[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(3): 168-171.

ZhengHuijun;TaoZenghou;FangRenli;ChangBaoyu;ZhangYulin;PaulTurner. APPLICATION OF IMMUNOCHROMATOGRAPHIC TEST FOR DIAGNOSIS AND SURVEILLANCE OF BANCROFTIAN FILARIASIS[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1998, 16(3): 168-171.

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