中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (5): 269-272.

• 论著 • 上一篇    下一篇

用rDNA研究海南省大劣按蚊的种型分类地位

徐建农; 瞿逢伊   

  1. 第二军医大学寄生虫学教研室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-10-30 发布日期:1997-10-30

INVESTIGATION OF THE SPECIESSTATUSOF ANOPHELES DIRUS( DIPTERA:CULICIDAE) FROM HAINAN PROVINCE USING rDNA

Xu Jiannong; Qu Fengyi   

  1. Department of Parasitology; Second Military Medical University; Shanghai 200433
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-10-30 Published:1997-10-30

摘要: 目的 :为了进一步确定海南省大劣按蚊的种型分类地位。方法 :以采自海南省的大劣按蚊实验室品系、野外现场捕蚊及采自泰国的大劣按蚊 A种 ( AFRIMS实验室品系 )为材料 ,用 PCR扩增比较海南省与泰国大劣按蚊的 r DNA第二内转录间隔区 ( ITS2 )序列。结果 :测出序列 84 1bp,包括 ITS2及两侧的 5.8S和 28S编码基因的一小部分序列 ,ITS2长 716bp,海南省与泰国大劣按蚊序列相同 ,未发现种内或种群内变异。结论 :证实海南省存在大劣按蚊 A种 ,与以往染色体核型分析及蚊卵扫描电镜的观察结果一致。

关键词: 大劣按蚊, 核糖体核糖核酸, 第二内转录间隔区

Abstract: AIM:To ascertain the species status of the Anopheles dirus from Hainan
Province,Chi- na.METHODS:The nucleotide sequence of the second internal transcribed spacer(ITS2
) of PCR- amplified r DNA was determined for the An.diruss pecimens which included 2 individu-
als of the species A colony (AFRIMS) from Thailand and 5 individuals from Hainan Province.RESUL
TS:A841 bp fragment was amplified from single mosquito.The fragment included the ITS2 and small
portions of flanking 5.8S (96bp) and 28S (29bp) genes. The ITS2 was 716 bp in length, the
sequence was identical for all 7 individuals from both Hainan Province and Thailand. No evidence
of intraspecies or intrapopulation variation was detected in the ITS2 and flanking regions.
CONCLUSION: The result suggests the existence of An. dirus A in Hainan Province, which was in
agreement with previous studies from cytogenetic analysis and egg characteristics determned by
scanning electron microscopy.

Key words: Anopheles dirus, ribosomal DNA, second internal transcribed spacer