中国寄生虫学与寄生虫病杂志 ›› 1996, Vol. 14 ›› Issue (4): 262-265.

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日本血吸虫和曼氏血吸虫减毒尾蚴免疫小鼠淋巴细胞表型的动态

石佑恩,吕芳丽   

  1. 同济医科大学寄生虫学教研室
  • 出版日期:1996-11-30 发布日期:2017-01-05

DYNAMIC STUDY ON PHENOTYPE OF LYMPHOCYTES IN MICE AFTER VACCINATION WITH RADIATION ATTENUATED CERCARIAE OF SCHISTOSOMA JAPONICUM AND S.MANSONI

  • Online:1996-11-30 Published:2017-01-05

摘要: 目的:宿主经照射减毒血吸虫尾蚴免疫后能获得显著的抗攻击感染的抵抗力。本文探讨紫外线减毒尾蚴免疫小鼠淋巴细胞表型的动态变化。方法:小鼠分别感染减毒日本血吸虫和曼氏血吸虫尾蚴(500条/只),对照组分别感染两种正常尾蚴(200条/只),免疫后第3、5、7、10、14和21d各组剖杀6-7只,摘取两侧腋窝淋巴结和脾脏分离细胞,采用双染色免疫荧光标记的单抗及FACScan流式细胞仪进行细胞表型分析。结果:免疫小鼠腋窝淋巴结细胞总数增加,第10d达高峰,其中尤以T细胞显著,而T细胞总数增加主要是由于CD+4T细胞的增加,而不是CD+8T细胞。与对照组相比,免疫组的反应是非常显著。结论:CD+4T细胞在紫

关键词: 血吸虫, 疫苗, 淋巴细胞表型, 保护性免疫, 小鼠

Abstract: AIM:Vaccination with radiation attenuated cercariae of schistosomes induced partial resistance in several hosts against challenge with normal cercariae.In the study,phenotype of lymphocytes in mice after vaccination with uv attenuated cercariae were investigated.METHOD:Female NMRI mice were used, each received either 200 untreated or 500 attenuated cercariae of S.japonicum or S.mansoni 6-7 mice of each group were killed at 3 d,5 d,7 d,10 d,14 d and 21 d after infection or vaccination.The axillary lymphnodes and the spleens were removed.Cells were prepared separately and were analysed by double colour immunofluorescence with conjugated monoclonal antibodies in a FACScan cytofluorimeter at 488 and 560 nm. RESULTS:Immunization with 500 attenuated cercariae was followed by a three to four fold increase in cell number in axillary lymph nodes,irrespective of schistosome species.In both cases,CD + 4 as well as B cell numbers increased,reaching peak levels at around 10 days;while CD + 8 cells did not appreciably vary over the three weeks of observation.CONCLUSION:On the basis of this study it is suspected that CD + 4 cells might play a significant role in the protective immunity elicited by uv attenuated cercarial vaccine.

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