中国寄生虫学与寄生虫病杂志 ›› 1994, Vol. 12 ›› Issue (2): 138-141.

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单克隆抗体对溶组织内阿米巴吞噬红细胞的影响

程训佳,黄美玉   

  1. 上海医科大学基础医学院寄生虫学教研室
  • 出版日期:1994-05-31 发布日期:2017-01-06
  • 基金资助:
    日本松前国际友好财团和OhyamaHealthFoundation的资助,上海医科大学学校基金

EFFECTS OF MONOCLONAL ANTIBODIES ON PHAGOCYTOSIS OF ENTAMOEBA HISTOLYTICA TO HUMAN ERYTHROCYTES

  • Online:1994-05-31 Published:2017-01-06

摘要: 用具有抗细胞膜活性和抗细胞核、胞浆活性的单克隆抗体3F7和4G6与致病性溶组织内阿米巴HM-1∶IMSS株滋养体37℃孵育,在孵育即刻、5min、10min加入红细胞,检测滋养体吞噬红细胞的能力,并以正常小鼠血清和抗克氏锥虫抗体TCE04作为对照。结果,经单克隆抗体3F7孵育即刻的HM-1∶IMSS虫株滋养体吞噬红细胞能力明显减弱(P<0.001),而4G6抑制滋养体吞噬红细胞的作用不如前者明显。提示,抗细胞膜单克隆抗体对抑制滋养体吞噬红细胞有显著作用,但随着时间的推移,抑制作用明显减弱。

关键词: 溶组织内阿米巴, 单克隆抗体, 表面抗原

Abstract: Two monoclonal antibodies 3F7 and 4G6 were produced respectively against plasma membrane and nucleus cytoplasm of Entamoeba histolytica .The erythrocytophagous quantification and ability of pathogenic strain HM 1∶IMSS were evaluated by microscopy immediately at 5 min and 10 min after incubation with McAb 3F7 and McAb 4G6. Sera from normal mice and monoclonal antibody TCE 04 against Trypanosoma cruzi were prepared as negative controls.The McAb 3F7 showed stronger inhibitory action on the erythrocytophagous effect of HM 1∶IMSS than the McAb 4G6.The result suggested a significant inhibition( P 0.001) on erythrocytophagous effect of amoebic trophozoites in the presence of McAb 3F7. This inhibitory action appeared to have a decreasing tendency with the elapse of incubation time.

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