中国寄生虫学与寄生虫病杂志 ›› 1994, Vol. 12 ›› Issue (2): 107-110.

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杜氏利什曼原虫抗原基因在大肠杆菌内表达的初步研究

敬保迁,胡孝素   

  1. 华西医科大学寄生虫学研究室
  • 出版日期:1994-05-31 发布日期:2017-01-06
  • 基金资助:
    国家自然科学基金

PRELIMINARY STUDY ON EXPRESSION OF LEISHMANIA DONOVANI ANTIGEN GENE IN E.COLI

  • Online:1994-05-31 Published:2017-01-06

摘要: 以λgt11噬菌体为载体,杜氏利什曼原虫前鞭毛体基因组DNA为靶片段构建基因文库,并以杜氏利什曼原虫前鞭毛体全虫制备的高价兔免疫血清为探针,对文库进行筛选,获一持续阳性表达克隆,其表达肽段经Western-bloting证实分子量为39kDa,与β-半乳糖苷酶分子呈非融合状态存在。

关键词: 杜氏利什曼原虫, 基因表达, 抗体探针

Abstract: We constructed previously the expressing library of Leishmania donovani genomic DNA with λgt11 as vector.In this paper, 2×10 4 phages were plated on E. coli Y 1090r , and screened with a rabbit antiserum prepared by immunization with Leishmania donovani promastigotes.Bound antibodies were detected using alkaline phosphatase labeled anti rabbit antibodies.A positive expressing clone was detected and transferred into E.coli Y 1098r to prepare lysate,a 39 kDa polypeptide in E.coli Y 1089r lysate was recognized by anti Leishmania donovani serum.The result indicated that the 39 kDa polypeptide which was not fused with the major portion of β galactosidase existed disconnectedly.This finding remained to be further studied.

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